phenyaline slant. When ferric chloride was added to phenylalanine deaminase tube, it turned into army green color( positive result) which indicated that unknown microorganism contains phenylalanine deaminase enzyme. On the other hand, no color change in the slant indicates negative result for this test. The choices between two…………………. was narrowed down By performing this test. The positive result for phenylalanine deaminase indicated the unknown microorganism #404 is P.vulgaris. To check…
GENTAMICIN The drug selected for study is Gentamicin. Gentamicin is an aminoglycoside antibiotic composed of a mixture of related Gentamicin components and fractions and is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms. Isolation and Preliminary studies 2 demonstrated that it is a mixture of basic, water soluble antibiotic containing the aminocyclitol 2-deoxystreptamine and two additional amino sugars. Chromatographic separation of…
Proteus Mirabilis Proteus mirabilis is defined by the slide share website as a disease caused by microbial invasion of the genitourinary track that extends from the renal cortex of the kidney to the urethral meatus. Proteus mirabilis can be laboratory diagnosed by the specimen collection, transported and storage and laboratory methods, but the laboratory methods that would be focused on is specimen collection. It is often the first determine diagnosis and treatment which would give a more…
DISCUSSION: Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates. To examine the antimicrobial activity of those isolates, in vitro inhibition by cross…
is essential (Bush 2014). One recent study shows 50% of HIV/AIDS patients had severe sepsis at ICU admission or during the ICU stay. An astounding 90% of nosocomial infections were the source of sepsis and the microbiology of infections was mostly gram-negative rods Serratia marcescens (three) made up 7% (Japiassú…
of a body part effect the bacteria population present on the said part? What has more bacteria the inside of a person's mouth or a person's index finger? My biology class is learning how to conduct experiments, and last week for our experiments we were broken into groups and given instructions to swab any two different objects. After swabbing our two objects, we were then suppose to wipe each swab onto a divided petri dish in order to see which object had more bacteria. My group could have…
The aim of this experiment is to identify and classify of an unknown bacterial isolate. Morphological and Biological test were preformed to identifiy the isolates characteristics. The following characteristics were tested: gram-stain, motility, morphology, hemolysis, lactose fermetation, mannitol fermentation, osmotolerance, aesculin hydrolysis, starch hydrolysis, gelatine hydrolysis, amino acid decarboxylation, lipid hydrolysis, lactose fermetation, casein digestion, catalase enzyme production,…
Materials and Methods Materials used in experiment 1.1A: Nutrient agar plate Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an…
used to identify lactose-fermenting coliforms. This medium is typically used to test dairy products and water. The medium contains lactose which acts as a carbohydrate source, bile salts and crystal violet that act as an inhibitory agent towards gram-positive microorganisms and neutral red is added to serve as a pH indicator, microorganisms lower the pH which results in the colonies turning red. Some of the organisms that grow on this medium are: Escherichia coli ,Enterobacter aerogenes and…
previous section we found the following results. The effect of acceleration under the influence of additional mass. Data from our force lab. Column 1-3 shows the results gathered in the first trial from 50, 20 and 10 gram weights. Column 4-6 shows the results gathered from 50, 20 and 10 gram weights when an additional 500g was added to the…