Gram-positive bacteria

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    Gram-Negative Bacteria

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    Gram positive: First, the bacteria sample is placed on a glass slide and heated only to the point of rendering it innocuous in terms of being infectious to the handler. Next, the bacteria sample is treated with a gentian violet-iodine solution for up to sixty seconds. The slide is then gently rinsed under clean water and the Gram solution is applied, which is a mixture of iodine and potassium iodide diluted in water. This step triggers a reaction to the gentian violet compound. Initially, the…

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    Biochemical Unknown Lab

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    an unknown bacteria by using first the Gram Stain and based on the results of the gram staining, several biochemical tests can be performed to identify with accuracy the unknown bacteria. II. Identification Bacteria are divided into Gram Positive and Gram Negative based on the chemical composition of the cell wall. Gram stain, a differential stain, can be used to identify the shape, arrangement, and chemical composition of the microorganisms. In the chemical composition of the Gram stain, we…

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    Catalase Test Lab Report

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    During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For…

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    Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…

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    Gram Stain Test Lab Report

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    I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

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    consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding…

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    Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…

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    were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria from the compound microscope which…

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    Gram Stain Lab Report

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    When finding out unknown bacteria you have to go through several steps to figure out what it could be. I was given unknown bacteria number 28 and asked to run different testing to identify exactly what this bacterium is. I ran 6 test to determine my unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have. A gram stain is one of the most…

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    Indole Test Lab Report

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    partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K.…

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