Gram-positive bacteria

consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding…

I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

Gram positive: First, the bacteria sample is placed on a glass slide and heated only to the point of rendering it innocuous in terms of being infectious to the handler. Next, the bacteria sample is treated with a gentian violet-iodine solution for up to sixty seconds. The slide is then gently rinsed under clean water and the Gram solution is applied, which is a mixture of iodine and potassium iodide diluted in water. This step triggers a reaction to the gentian violet compound. Initially, the…

When finding out unknown bacteria you have to go through several steps to figure out what it could be. I was given unknown bacteria number 28 and asked to run different testing to identify exactly what this bacterium is. I ran 6 test to determine my unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have. A gram stain is one of the most…

During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For…

an unknown bacteria by using first the Gram Stain and based on the results of the gram staining, several biochemical tests can be performed to identify with accuracy the unknown bacteria. II. Identification Bacteria are divided into Gram Positive and Gram Negative based on the chemical composition of the cell wall. Gram stain, a differential stain, can be used to identify the shape, arrangement, and chemical composition of the microorganisms. In the chemical composition of the Gram stain, we…

Organisms present on the Gram film were obtained from patient sputum. This suggests that the organisms can be present either in the airways or in the lungs. Gram film shows Gram-positive (purple- dark colour) elongated oval shaped (cocci) cells. Bacteria are present as diplococci (pairs of cocci), nevertheless they also appeared as single cells and short chains of few cells (four cells chain is visible at the Gram film). Morphological appearance of bacteria, Gram stain and presence of the…

partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K.…

Contamination by Site While the previous data set is useful in showing the overall prevalence of pathogenic microbes on computers, it may be useful to further examine variations in contamination levels between different departments. This would allow one to determine which departments face the most risk and require the most changes. In the study conducted by Po-Liang et al. (2009), no significant differences in pathogenic contamination rates between ICU workstations and non-ICU workstations (7.4%…