Gram-positive bacteria

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    Gram + Cocci Lab Report

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    Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

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    Unknown Bacteria Report

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    correctly identify two unknown bacteria. Organism number thirteen is Escherichia coli. On the first day, I conducted a gram stain and noticed that the bacteria were pink, meaning that the bacteria are gram negative. I also recognized that the bacteria were rod shaped therefore they were bacillus. I also noticed that the arrangement appeared to be single celled. I also put the bacteria on a streak plate. On the second day, I did another gram stain and the bacteria were again pink and bacillus or…

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    Gram staining is a technique where certain stains are used to identify bacteria. It was discovered in 1884 by Hans Christian Gram where he used stains to differentiate bacterial cells in diseased lung tissue. Gram staining has evolved over time to become a trusted and common diagnostic tool used in clinical and research settings. Gram stains are mainly used to identify unknown bacteria. It is often the first test used on an unknown sample of bacteria. For medicinal uses gram staining is commonly…

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    Microorganisms

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    specimen thrive in aqueous environments, therefore they are translucent and when mounted on a microscope the light passes through them making it impossible to see the specimen. Equally as important as this is that the viewer is aware of what type pf bacteria is being utilized. Both of these criteria in a lab are met with the use of a staining procedure which allows for greater contrast between the mostly translucent cells and light used by the microscope. Staining is a critical step when…

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    The T-Streak Method

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    When trying to figure out the cellular morphology and arrangement the T-streak method is used to get isolated colonies from a sample of a bacteria. To begin the process a petri plate which has a layer of agar is divided into three sections in a T form. When preforming an experiment aseptic techniques are used between each step, therefore you flame the loop before and after every step. A sample is taken from a culture with an inoculated loop, then is spread in the top section of the plate in…

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    Essay On Nitrate Test

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    determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest bacteria to conduct the tests. Thus, this process allowed the unknown bacteria to grow and maintain. Before a…

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    Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria is seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple…

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    Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple…

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    Gram Staining

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    After my gram staining I was able to comfortably eliminate all of the gram negative results. This encouraged me in knowing that I was able to execute an experiment efficiently enough to eliminate some of my choices. I was also able to eliminate all of the streptococcus bacteria because my bacteria was in clusters and not in a chain arrangement. Gram staining also helped me in eliminating the bacilli because my interpretation of the observation was that my unknown bacteria was spherical in nature…

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    Mixed Unknown Lab Report

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    determine two bacteria in a given broth. For this experiment we were provided with an unknown broth, along with the supplies and media to perform the needed tests that would determine what bacteria the broth contained. We were also provided with a chart of possible bacteria. All tests were preformed with aseptic and sterile techniques. After being given the broth, using sterile methods I streaked a TSA plate using the quadrant method with the given broth, in hope to grow the bacteria in…

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