Genomics

Determination SDS-PAGE Characteristics by Analyzing Proteins Pratik Patel April 7, 2015 Lab section: Tuesday 9:30 AM (Section 1) Lab partners: Tahia Mostafiz TA: Fei Chai Introduction The main reason of performing this experiment is to learn on how to load and run SDS-PAGE gels. The SDS-PAGE stands for sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sodium dodecyl sulfate is a detergent, which can dissolve hydrophobic molecules due to the negative charge that is attached…

Chase Rocker Savage 1101 Period 2 November 7, 2016 Animal Testing Strapped to a table with no means of escape, a rabbit squirms to avoid the toxic eye-drop being forced into its ultra-sensitive eye. It shrieks as the drops leave chemical burns on its eye, often blinding it in the process. It endures this pain, sometimes for over a week, as scientists poke and prod to rate the swelling and corneal opacity. All of this suffering is inflicted merely to determine if the product is acceptable for…

This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution…

Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

The often debated concept of Nature vs. Nurture has in recent years become less of a polarizing topic. With advancements in psychology and biology, the factors that go in to the etiology of certain disorders and diseases have become more clear, but also less clearly distinct from one another. The lines between what is caused by environmental factors or by biological factors are becoming more and more blurred. In particular, social anxiety acts as an excellent example of how these factors can…

The Indian subcontinent has served as a major corridor for the genetic diversity of its inhabitants. Modern-day human population dispersal routes is punctuated with delineating migrations that eventually led anatomically modern humans (AMH) to spread over most parts of the world within the past 100,000 years (Cavalli- Sforza, Menozzi and Piazza 1994; Lahr and Foley 1994). Migration waves are influenced by landscape traversed and thus, natural inter continental passageways acting as corridors…

As the world of science enters the realm of what was once science fiction, many important debates and discussions will serve to mold the world’s future. Thus enter the old science fiction flicks such as the 1997 film Gattaca, a movie ahead of its time with respect to its focus on parents’ eugenic policies that promoted the manipulation of their children’s genes. After viewing this film, it is difficult not to conclude that any form of gene editing technology only leads to a dystopian future in…

An insight into the methods of Conservation Genetics Understanding the need to use genetic methods in conservation biology will greatly aid in the process of bringing back any species that play an important role in their native habitat. Implementing genetic tools and methods in conservation programs helps to minimize the loss of genetic diversity, which allows populations to adapt to environmental changes and stressors. This helps to minimize the loss of the population numbers by reducing the…

Humanity has a long history of experimentation on animals, especially in the medical and cosmetic professions. Many people argue that humanity would not be where it is today if it were not for its reliance on animal experimentation. It is nigh unto impossible to deny that animal testing has ever been useful, although the argument could certainly be made that such a method was not always necessary. However, with advancements in medical and scientific technology, animal testing is no longer a…

Over the past decade exome sequencing has become a very useful tool in genetic testing. While there are many other methods used for genetic testing, exome sequencing has many advantages over them. Much like any other method, exome sequencing has a few practical limitations. Though this type of sequencing is a fairly new method, more recent research has found it extremely beneficial in aiding with genetic disease diagnosis as well as cancer studies. Through the analysis of exome sequencing,…