Chronological lifespan of S. cerevisiae mutants lacking telomere capping protein Cdc13 and Exo1
Lay summary
For over 25 years, yeast has been used as a model to understand cellular processes such as how; DNA is repaired and produced, also for understanding different aspects of the cell lifecycle and principle molecular processes in eukaryotes (the same type of cells that make up humans, animals and plants). Because yeast can be easily grown until it dies in a relatively short time, it is an excellent model for looking at how cells age.
One of the methods of recording how yeast cells age is by measuring their chronological lifespan (CLS). CLS measures how long yeast cells live without dividing. By altering the yeasts genes, which will prevent …show more content…
The use of yeast has already lead to the discovery of processes related to human aging.
This project will involve mutant strains of yeast that have had the genes producing proteins which cap the ends of telomeres (capping proteins) removed. Inconsistencies in CLS has been observed with mutant strains that have the same genotype (set of genes that make certain proteins) which are sourced from different origins. The aim of this project is to confirm this observation, and to demonstrate that this could be associated with high mutability of mutants which hide actual CLS phenotypes (expressed genes) of individual clones. It will allow further experimentations in CLS to become more accurate. The CLS of individual and bulk colonies will be measured to account for natural mutations and variations of the yeast.
This research could be linked to the vast majority of diseases which are related to aging via mechanisms of telomere maintenance. This research has the possibility of contributing to new treatments of these …show more content…
(Piper, 2006). Chronological lifespan is measured in the length of time a yeast cell population can remain viable in a post replicative state. This is achieved when the yeast population has exhausted the carbon present in the media and enters the quiescent G0 phase. Alternatively yeast cells can be isolated, stored in H2O and refrigerated to induce G0 arrest. Viability over time is measured in the ability of somatic yeast to restart mitosis in fresh growth medium. Keeping the cells in a buffered media, removes any inhibitory products e.g. acetic acid (which has been shown to reduce lifespan (Burtner et al, 2009), additional molecular pathways that are necessary in regulating chronological lifespan can then be investigated (Kaeberlein et al,
Lay summary
For over 25 years, yeast has been used as a model to understand cellular processes such as how; DNA is repaired and produced, also for understanding different aspects of the cell lifecycle and principle molecular processes in eukaryotes (the same type of cells that make up humans, animals and plants). Because yeast can be easily grown until it dies in a relatively short time, it is an excellent model for looking at how cells age.
One of the methods of recording how yeast cells age is by measuring their chronological lifespan (CLS). CLS measures how long yeast cells live without dividing. By altering the yeasts genes, which will prevent …show more content…
The use of yeast has already lead to the discovery of processes related to human aging.
This project will involve mutant strains of yeast that have had the genes producing proteins which cap the ends of telomeres (capping proteins) removed. Inconsistencies in CLS has been observed with mutant strains that have the same genotype (set of genes that make certain proteins) which are sourced from different origins. The aim of this project is to confirm this observation, and to demonstrate that this could be associated with high mutability of mutants which hide actual CLS phenotypes (expressed genes) of individual clones. It will allow further experimentations in CLS to become more accurate. The CLS of individual and bulk colonies will be measured to account for natural mutations and variations of the yeast.
This research could be linked to the vast majority of diseases which are related to aging via mechanisms of telomere maintenance. This research has the possibility of contributing to new treatments of these …show more content…
(Piper, 2006). Chronological lifespan is measured in the length of time a yeast cell population can remain viable in a post replicative state. This is achieved when the yeast population has exhausted the carbon present in the media and enters the quiescent G0 phase. Alternatively yeast cells can be isolated, stored in H2O and refrigerated to induce G0 arrest. Viability over time is measured in the ability of somatic yeast to restart mitosis in fresh growth medium. Keeping the cells in a buffered media, removes any inhibitory products e.g. acetic acid (which has been shown to reduce lifespan (Burtner et al, 2009), additional molecular pathways that are necessary in regulating chronological lifespan can then be investigated (Kaeberlein et al,