Paper #1 Analysis Worksheet Name/ID: JAE KUG 87672117
1. What is the main question this paper is asking? This should be a 1-2 sentence/phrase answer (complete sentences not necessary).
Can we identify and sequence genes on S. cervesiae are responsible for ethanol tolerance and production? Can we alter them using gTME to increase ethanol/glucose tolerance and have more efficient ethanol production?
2. Answer the following questions regarding the figures below. Answers should be 1-2 sentences/phrases at most (complete sentences not necessary).
Figure 1B
a. Why was this experiment done? (Purpose):
Identify which gTME mutants had the highest growth rates in presence of high glucose ethanol concentrations.
Quantify and compare gTME …show more content…
How was this experiment done? sp15-300 and taf25-300 mutants 6% ethanol and glucose concentrations for 20 hours and the improvement in growth rate was quantified and compared with yeast strains with wild-type plasmids of SPT15 and TAF15.
c. What were the results for the experiment? spt15-300 mutants had higher growth yields than the control, some as high as 13x spt15-300 had better growth than taf-300 mutants, which did not grow in 6% ethanol media
In absence of ethanol and glucose, the growth rate of spt15-300 and taf-300 mutants were near controls.
d. What conclusion is drawn from these results? spt15 mutants grew best in elevated concentrations of ethanol and glucose. Mutations present in different transcription-regulating genes will probably exhibit different changes in phenotype.
Figure 3A
a. Why was this experiment done? (Purpose):
Determine if up-regulated genes in spt15-300 mutants work alone or as a group to bring about increased ethanol and glucose tolerance
b. How was this experiment done?
Up-regulated genes in spt15-300 mutants knocked-out individually in mutant and normal yeast strains.
Tolerance to 5% ethanol, 60g/L tested by comparing knockout strains with spt15-300 mutant and strain with wild-type STP-15 containing
1. What is the main question this paper is asking? This should be a 1-2 sentence/phrase answer (complete sentences not necessary).
Can we identify and sequence genes on S. cervesiae are responsible for ethanol tolerance and production? Can we alter them using gTME to increase ethanol/glucose tolerance and have more efficient ethanol production?
2. Answer the following questions regarding the figures below. Answers should be 1-2 sentences/phrases at most (complete sentences not necessary).
Figure 1B
a. Why was this experiment done? (Purpose):
Identify which gTME mutants had the highest growth rates in presence of high glucose ethanol concentrations.
Quantify and compare gTME …show more content…
How was this experiment done? sp15-300 and taf25-300 mutants 6% ethanol and glucose concentrations for 20 hours and the improvement in growth rate was quantified and compared with yeast strains with wild-type plasmids of SPT15 and TAF15.
c. What were the results for the experiment? spt15-300 mutants had higher growth yields than the control, some as high as 13x spt15-300 had better growth than taf-300 mutants, which did not grow in 6% ethanol media
In absence of ethanol and glucose, the growth rate of spt15-300 and taf-300 mutants were near controls.
d. What conclusion is drawn from these results? spt15 mutants grew best in elevated concentrations of ethanol and glucose. Mutations present in different transcription-regulating genes will probably exhibit different changes in phenotype.
Figure 3A
a. Why was this experiment done? (Purpose):
Determine if up-regulated genes in spt15-300 mutants work alone or as a group to bring about increased ethanol and glucose tolerance
b. How was this experiment done?
Up-regulated genes in spt15-300 mutants knocked-out individually in mutant and normal yeast strains.
Tolerance to 5% ethanol, 60g/L tested by comparing knockout strains with spt15-300 mutant and strain with wild-type STP-15 containing