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  • Bacillus Cereus Lab Report Answers

    were gram positive rods, I could tell they were positive by the purple color to them and the rod shape by the comparison provided in the lab manual (Wilkosz, Peterson). After I confirmed the results I performed the spore stain, which indicated that my sample was positive so it contained endospores. The sample should have shown that there was a red vegetative cell and a green stained endospore (Wilkosz, Peterson), but I must have over rinsed with water because the cells appeared clear with an outline, and the spores that were seen appeared as black dots. c. The first test performed was the gram stain, the purple color indicated that it was gram positive, this removes all gram negative possibilities including Pseudomonas aeruginosa, Klebsiella pneumonaie, and Proteus vulgaris. Since I also observed that the bacteria were rod shaped, it excluded the cocci specimens Staphylococcus aureus and Streptococcus pyogenes. After performing the spore stain and noticing that there were spores, which concluded that it was Bacillus cereus. d. I had Unknown plate #12 which I determined…

    Words: 1479 - Pages: 6
  • Endospore Research Paper

    The endospore is a quiescent and tough structure that is form by some gram-positive bacteria that allows them to survive in harsh and extreme environments. Because only some genera of bacteria produces the endospore, its identification is important in helping us identify some bacteria. 2. What type of eukaryotic cells did we observe and how were they different from the prokaryotes we have looked at? (Be sure to include ways they are different internally as well as any observable traits) The…

    Words: 448 - Pages: 2
  • Compare And Contrast Prions And Mycobacterium

    Prions, endospores, and mycobacterium all are extremely resistant to antimicrobial treatment. Prions are extremely small, even smaller than viruses. Heat or formaldehyde aren’t very effective against prions. Endospores are extremely resistant to harsh conditions as well. They can survive heat up to 100 degrees C, drying, radiation, and majority of chemicals. Mycobacterium are extremely resistant due to their waxy cell wall. This cell wall blocks many things from entering the bacteria, making it…

    Words: 398 - Pages: 2
  • Antimicrobial Properties Of Silver Nannoparticles

    They investigated the synthesis of silver nanoparticles by the spore crystal mixture of Bacillus thuringiensis (Bt). Bt had been used because of its ease of availability and lack of seasonal dependence. The Bt strain IS1 was isolated from the soils of Bikaner, Rajasthan and was added to a mixture of silver nitrate to form silver nanoparticles [5]. The AgNPs synthesized were found highly toxic against many different multi-drug resistant human pathogens: Escherichia coli, Pseudomonas aeruginosa,…

    Words: 1753 - Pages: 8
  • Microbiology Lab Report

    cell morphology tests were concluded, five more tests were used to determine the identity of the unknown organism. Endospore Stain Although the gram stain established unknown organism’s shape and size, it cannot reveal the presence of an endospore. Endospores are harder to stain due to their resistance to heat and chemicals via the layer of keratin, allowing them to survive in harsh conditions (2). The identification of dormant endospores establishes identification of the unknown organism…

    Words: 840 - Pages: 4
  • Unknown Lab Report Essay

    negative as well as rod shaped. With this information, all gram negative microbes could be eliminated. What was left was a list of gram positive microbes. Families Staphylococcaceae, Enterococcaceae, Streptococcacaeae, and Micrococcineae could also be eliminated from the list of gram positive microbes because all of these families are cocci shaped. Another test that helped determine the identity of unknown microbe #7 was the endospore staining. This test was used for identifying whether or…

    Words: 808 - Pages: 4
  • Microscope And Hanging Lens

    staining which are, simple staining, negative staining, endospore staining, gram staining and capsule staining. Simple staining can be used to determine cell shape, size, and arrangement. Due to its name, the simple stain is a very simple because of the procedure that involving only one stain. In this experiment, we have observed the staphylococcus aureus sp. with simple staining using methylene blue as dye. Due to the methylene blue, we can see that the result of the cell bacteria stained blue…

    Words: 1350 - Pages: 6
  • Lab Report: Phalyco-Chemical Characteristics Of Chemical Acid

    pipette Titrimetric 14 BOD Burette and pipette Titrimetric 15 COD Burette and pipette Titrimetric 2. ISOLATION OF MICROBES: The isolation microbes were done from ETP and the effluent waste water. Serial dilution method was done for obtaining pure colonies. Liquid culture was inoculated and spread onto an agar plate. After that streaking was done and the plate was incubated for 24-48 h for bacterial growth. Fig. 3 Serial dilution 3. CHARACTERIZATION OF THE BACTERIA:- Characterization…

    Words: 1564 - Pages: 7
  • Unknown Lab Report

    sterilize the loop for a few seconds and hold it steady to cool, next pass the tube in front of the incinerator before dipping the cooled sterilize loop into the broth. It is important to pass the tube in front of the incinerator again before capping it, this will help prevent contamination to occur. Streaking plate also requires the use of aseptic technique, but the loop is flamed every single time before moving onto the next quadrant, this will help with an isolating colony. Not only does…

    Words: 1511 - Pages: 6
  • Unknown 15 Archetype

    Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella, Erwinia, Veillonella, and Flavobacterium (5). Bacillus, Lactobacillus, Listeria, Kurthia, and Arthrobacter were eliminated because they did not match the coccoid shape of Unknown 15 (5). The catalase test was the next designated test in the identification of Unknown 15. Unknown 15 tested negative for the enzyme catalase as it did not form gas bubbles after the addition of hydrogen peroxide to the sample. Thus, all…

    Words: 1308 - Pages: 6
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