The endospore is a quiescent and tough structure that is form by some gram-positive bacteria that allows them to survive in harsh and extreme environments. Because only some genera of bacteria produces the endospore, its identification is important in helping us identify some bacteria.
2. What type of eukaryotic cells did we observe and how were they different from the prokaryotes we have looked at? (Be sure to include ways they are different internally as well as any observable traits)
The type of eukaryotic cells that we observed were yeast cells. Under the microscope, the only observable differences between the yeast cells and the prokaryotic cells that we have looked at, is that the yeast cells are larger and some cells appear to be budding. When viewed under Phase Contrast, the yeast cells shows different level of contrast on its membrane, possibly due to the organelles within the cells. Other differences that were not observable under the microscope, is that yeast, being a eukaryotic, contains membrane bound organelles, an endomembrane system, as well as a cytoskeletal system. The ribosomes of yeast cells are also larger at 80S, than the …show more content…
The specimen is kept moist the entire time by adding drops of 5% malachite green onto a piece of bibulous paper that is covering the specimen. Afterward the bibulous paper is removed and the specimen is rinsed with DI water before being counterstained with safranin. Because of the endospore layer, endospore staining requires a longer period of time for the specimen to be stain and heat is used to drive the stain into the cells. The endospore layer also help retain the dye even after decolorized with DI water. Contrary, the malachite green will be washed of the cells without an endospore and take up the counterstain more easily making them visibly different under the
2. What type of eukaryotic cells did we observe and how were they different from the prokaryotes we have looked at? (Be sure to include ways they are different internally as well as any observable traits)
The type of eukaryotic cells that we observed were yeast cells. Under the microscope, the only observable differences between the yeast cells and the prokaryotic cells that we have looked at, is that the yeast cells are larger and some cells appear to be budding. When viewed under Phase Contrast, the yeast cells shows different level of contrast on its membrane, possibly due to the organelles within the cells. Other differences that were not observable under the microscope, is that yeast, being a eukaryotic, contains membrane bound organelles, an endomembrane system, as well as a cytoskeletal system. The ribosomes of yeast cells are also larger at 80S, than the …show more content…
The specimen is kept moist the entire time by adding drops of 5% malachite green onto a piece of bibulous paper that is covering the specimen. Afterward the bibulous paper is removed and the specimen is rinsed with DI water before being counterstained with safranin. Because of the endospore layer, endospore staining requires a longer period of time for the specimen to be stain and heat is used to drive the stain into the cells. The endospore layer also help retain the dye even after decolorized with DI water. Contrary, the malachite green will be washed of the cells without an endospore and take up the counterstain more easily making them visibly different under the