I. The most basic unit of life is the cell. All living things are composed of cells. Cells may only from through a process of division and replication of previously existing cells. Cells are microscopic; however their structure is complex. All types of cells possess a plasma membrane which separates its contents from its surroundings. . There are two types of cells: prokaryotic and eukaryotic. a. Prokaryotic cells are simpler than eukaryotic cells. Within prokaryotic cells are bacteria cells…
Since reverse transcription is non-specific to RNA molecules, there is the possibility of having mRNA that code for proteins other than Actin. To solve this problem, there were specific primers that were used in PCR, primers 1 and 2 (forward and reverse, respectively); these primers are specific for gene of interest. The primers are Actin forward (1) and Actin reverse (2). During preparation of the reaction, most of the time the reagents are left on ice, this is to prevent the primers and…
sodium ions exchanging start to spread both sides of muscle cell membrane to form the action potentials, and long with the cell membrane so that both sides of the terminal cistern will release Ca2+, Ca2+ and troponin binding to change tropomyosin, let actin exposed binding site to the cross bridge.…
for it to be looked under the confocal microscope to view the mitochondria and nucleus. After staining the slice they treated the tissue with 4% formaldehyde and 0.05% ethidium bromide in a NaCl solution. In some of the experiments it was evident actin was visible under the fiber…
To begin, the role of the ACH receptor is vital to biology. An incoming action potential to an alpha motor neuron causes acetylcholine or ACH, at the end plate. The ACH binds to ACH receptors on the sarcolemma causing a depolarization through sodium influx. In your body, calcium performs a number of basic functions. The body uses around 99 percent of its calcium to keep bones and teeth strong, as a result, supports skeletal structure and function. The rest of the calcium in your body plays…
Collagen Collagen functions as a structural component. Collagen is the most abundant protein in the human body and is the substance that holds the whole body together. It is found in the bones, muscles, skin and tendons, where it forms a scaffold to provide strength and structure. Lupus is a collagen vascular disease that causes unique symptoms in each patient. Additional symptoms can include shortness of breath, chest pain, headaches, and dry eyes. People with lupus may have long periods of…
muscle is the 427kDa isoform. Dystrophin is made up of four domains: an N-terminal, a rod domain composed of 24 triple helix repeats and 4 hinges, a cysteine-rich domain and a C-terminal. The N-terminal of the protein binds to actin filaments and acts as a bridge between the actin cytoskeleton and the extracellular matrix via interactions with the dystroglycan complex (DGC) in the sarcolemma in muscles (5). Dystrophin helps to increase membrane stability and prevent the sarcolemma from…
Brazilian Jiu-jitsu Brazilian Jiu-jitsu, or BJJ, is a style of martial arts, modified from the ancient Japanese art of Jiu-jitsu by the Gracie family and others, based on using an opponent’s momentum and leverage against them for defense. It involves take-downs and grappling, and has become a large sport that can be seen incorporated in the televised sport Mixed Martial Arts, or MMA. BJJ is a high intensity sport that involves endurance training as well as resistance training and explosive…
Determining phylogenetic relationships of marine invertebrate and vertebrate using variation in muscle proteins of different genera; Ostreidae , Teuthida, Pectinidae, Nephropidae, Caridea, Brachyura and Oncorhynchus. Introduction The fundamental core of all biology emanates from evolution, as the great evolutionary biologist Theodosius Dobzhansky once said "Nothing in biology makes sense except in the light of evolution"(1973). Therefore, studying the phylogenetic relationships of different…
Structure of Tight Junctions and their Dynamic Functions in Health and Disease Tight junction (TJ) discovery in the 1960s helped determine the nature of paracellular transport while the discovery of their constituting proteins subsequently improved understanding of TJs’ dynamic structure. Their role can be appreciated particularly in epithelial cells and when their formation is compromised; I aim to briefly explore the cause of Crohn’s disease to illustrate this. Furthermore, I will outline the…