Actin

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    Dna Synthesis Lab Report

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    Objective: We started by isolating a DNA plasmid from a bacterial culture for cloning. Then, we reverse transcribed mRNA to amplify it in a polymerase chain reaction. Finally, we amplified a specific gene (actin) through PCR. Protocol: A. Isolation of Plasmid DNA from a Bacterial Culture: We were provided with a tube containing 1 ml of bacterial culture. We centrifuged it for 5 minutes at 10000 g. Then, we removed the supernatant by pouring it off and we used a small chem wipe to remove any…

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    spreading velocity of KO platelets suggests that loss of Calponin hinders actin polymerization, leading to a delay in response to the contact with collagen. These results indicate Calponin plays an important role in platelet adhesion and clot formation and supports our hypothesis. Lack of Calponin in platelets delays platelet activation and mobility. This supports our original hypothesis that Calponin is an important part of actin myosin filament in platelets and plays a major role in platelet…

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    1. What are the functional classifications for the biceps and triceps muscles in the bicep curl movement? What type of contractions are the biceps and triceps performing during a bicep curl? There are two functional classifications for the biceps and triceps muscles, which are the following: agonists and antagonists. The agonist generates the most powerful force in the biceps, in comparison to the triceps. The Triceps focuses on protecting the muscles from hyperextending the arm, by using the…

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    1. The resting membrane potential is the potential difference across the cell membrane of excitable cells such as nerve and muscle cells. The 3 major factors that establish a resting membrane potential are as follows: (1) Non-diffusible anions: The resting membrane potential is influenced by the presence of large non-diffusible anions (proteins, nucleic acids and organic phosphates) inside a cell. The presence of large non-diffusible anions inside a cell attracts a large number of cations and…

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    1. The peripheral nervous system, which is divided into the sensory and motor divisions, is involved in physical sensation. Sensory impulses move though the body by being stimulated by a receptor in the skin. It then travels to the sensory neurons and through the afferent fibers, were it will end up at the spinal cord as well as the brain. 2. The motor division of the nervous system are involved in skeletal muscle movement. Motor impulses move through efferent fibers. A motor unit is a motor…

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    “A muscle is a tissue composed of cells or fibres, the contraction of which produces movement in the body.” (Dictionary) There are three different types of muscle; voluntary skeletal muscle, involuntary smooth muscle and cardiac muscle. Muscles have many different functions within the body. One function of muscle is movement. It is the only tissue in the body which is able to contract and therefore can move more than other body parts. Another function is the maintenance of posture. The muscles…

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    Hypertrophy means that the muscles size will increase. Hypertrophy happens when the amount contractile proteins (Actin & Myosin) increase inside the muscle cells so the way in which the can contract is far greater.the amount of fibres in the muscles remain the same mount. Statistically males have a greater chance in muscle bulk due to higher levels of the hormone testosterone. As it relates to skeletal muscle, hyperplasia defines muscle growth due to an increase in the quantity of muscle…

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    Zingerone Essay

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    2. Materials and methods 2.1. Chemicals Zingerone was obtained from Alfa Aesar, Heysham, UK. Cisplatin, Bradford reagent, trichloroacetic acid (TCA), thiobarbituric acid (TBA), reduced glutathione (GSH), glacial metaphosphoric acid (Gmpa), 5,5ˊ-dithiobis-(2-nitrobenzoic acid) (DTNB), proteinase K and agarose were purchased from Sigma-Aldrich Co, MO, USA. All other chemicals and solvents used were of the highest purity grade available. 2.2. Animals Sixty Adult male albino rats (180-200g) were…

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    Cytoskeleton Lab

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    cell’s bones, muscles, and ligaments by supporting cellular structures and providing the machinery to generate various cell movements (Hopkins, 2010). There are three types of rods in the cytoskeleton, and each filament has specific role microfilaments Actin filaments are considered to be the most abundant in the cell; they also create contractile forces (Hopkins, 2010). Secondly, the Microtubules help with the duplication and the division of in the cells. Lastly, Intermediate filaments are use…

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    cloning, etc. (Kelly, 2016). This allows for the ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific section of DNA 9 specific steps had to be conducted and they are as follows: extraction/purification of DNA, amplify the gene utilizing PCR, ligate the product from PCR into plasmid…

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