Lens Fiber Differentiation Experiment

1942 Words 8 Pages
Lens Fiber Differentiation and its effects on certain organelles.

This writing exercise analyzes two separate but related scientific articles, “The fate of the Golgi Apparatus and the Endoplasmic reticulum During Lens Fiber Cell Differentiation” and “Coincident Loss of Mitochondria and Nuclei During Lens Fiber Cell Differentiation” both which analyze and study Lens Fiber Differentiation. The writing assignment will be based on four sections:(1) Background of the research (2) Methods used to approach the study (3) Results obtained; and, (4) Concluding both articles. Background Before one starts this scientific journey it is apparent the individual should have some basic knowledge regarding what lens fiber is and which part of the
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Lenses and vitreous body were removed from the embryos from day 6-20. The vitreous body and lens are then transferred to a petri dish where they would be separated by doing a careful disection. Dr. Mark Adeelman donated the rhodamine-123 for the study which was used a methanol stock. This stock was used for the fluorescent probes. In order to be able to examine the lens with the confocal microscope one needs to stain it with the fluorescent dye. This process didn 't seem to work accordingly therefore another method was adapted lens slicing. Article two states that although cell on the outer layer of the slice was being damaged there were tons of cells located deep inside the cell that were able to examine. Rhodamine-123 was also used in this process. once the lens was stained with the rhodamine-123 it would enter stain the mitochondrial matrix. Next they rinsed the lenses with saline solutions for it to be looked under the confocal microscope to view the mitochondria and nucleus. After staining the slice they treated the tissue with 4% formaldehyde and 0.05% ethidium bromide in a NaCl solution. In some of the experiments it was evident actin was visible under the fiber

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