Restriction enzyme

Ultrasound and meat quality The quality of meat is based on aroma, taste, appearance, texture and juiciness. Texture is the most important factor in determining the quality of meat as per consumer behavior. There are several factors that affect texture such as the tenderness of the meat, juiciness and the degree of maturation. Tenderness The quality of meat as a result of using Traditional tenderising methods is poor. This includes mechanical, enzymatic and chemical methods…

During the phase and process of the bacterial unknown project, vast amounts of test were performed in order to determine an unknown species of bacteria. Within the many procedures of identifying the unknown bacteria, I was first given a tube with the unknown bacteria, from which later on I started subcultures by streaking bacteria from the original tube onto a tryptic soy agar (TSA) slant. Throughout the process of identifying the unknown, new subcultures were produced in order to use the newest…

Enzymes are catalysts to help facilitate chemical reactions (RSC 2004). They do not cause the reaction to occur, but they speed up the rate of reaction (RSC 2004). Each enzyme is a specific protein, meaning they work for the purpose of a particular reaction or substance (EUFIC 2016). This can help explain why they are essential in the role of digestion. The particular enzyme that we looked at in this experiment was salivary amylase. Salivary amylase is an enzyme that helps with the breakdown of…

basics of enzymes and how they work. We were able to perform a quantitative assay of the activity of an enzyme in a tissue extract using a spectrophotometer. Also, we had to organize the data that was provided into tables and graphs so we could have the ability to test a few hypotheses. For example, we tested the whether the rate of the reaction was influenced by enzyme concentration, whether the activity of the enzyme was influenced by temperature and also too see if the activity of the enzyme…

What is the necessity of blood glucose level chart? Description: Blood glucose level chart is of greater important especially to those patients who are diabetic in nature. The chart should be prepared in a proper manner so that you can get the highest benefits. Do you wish to make effective diabetes management? Well, in this case you just need to follow a proper blood glucose level chart. This chart cannot be prepared by self-efforts rather you have to take the help of any expert doctor who is…

Enzymes are substances produced by living organisms that act as catalysts to reduce the activation energy required for a chemical reaction. By lowering the activation energy, enzymes speed up the chemical reaction by providing an alternative action pathway (“Enzymes”). Enzymes are required in minute amounts because they remain unchanged after a reaction has occurred, so the same enzyme molecules can be reused over and over again. Since enzymes are proteins, they have active sites that speed up…

Discussion In this study, the Catechol enzyme was studied under the conditions of varying pH, temperature, substrate concentration, and enzyme concentration. In Figure 1, the data suggested that the trend was neither directly nor inversely proportional, but the highest activity rate was at 24°C. Most enzymes denatured at higher temperatures of approximately 40°C, which led to the inability to see any color change (Helms et al., 1998). At lower temperatures, the enzyme was somewhat efficient…

Introduction: The purpose of the tests (culture media, motility, enzymes, etc.) we preform on the numerous bacteria in lab is to show any biological or chemical characteristics of the bacteria that may help it survive or adapt in the wild, how it may be useful or hazardous to humans, and use as general knowledge to differentiate bacteria from each other. In the Culture Media test we can use solid and liquid media to grow multiple bacteria in lab. It can help us determine what processes the…

deprotonation of a serine residue, Zn(II) ion at M2 position then stimulates Ser102 to attack the phosphorus center of the substrate to initiate the leaving of an alcohol group and to form a phosphoseryl intermediate. This intermediate resembles E-Pi, an enzyme-phosphate complex, in Figure 2 and…

Enzymes and Metabolic Rates Introduction Enzymes are catalysts that speed up chemical reactions without being consumed or changed themselves. Each enzyme has a substrate that will match perfectly, almost as if it is a lock and key fit. If the substrate does not fit the enzyme only partial to no function will occur. Each enzyme is formed of polypeptide chains that wind and bend to form complex grooves and valleys. These grooves are the active sites. The active site is where the substrate will…