Restriction enzyme

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

What is a catalase? A catalase is an enzyme that destroys harmful substances inside your body, and is a form of protein. (Corsini, "What Is a Catalase?") What is an enzyme? Enzymes are substances that speed up chemical reactions. (Corsini, "What Is a Catalase?”) This experiment involved both of these two aspects. It was inferred before the experiment began that if the potato is placed in hydrogen peroxide at room temperature, then it will produce the most bubbling activity. The reason that the…

Initially, the protein extract solution was subject to ion exchange column chromatography using SP Sepharose Fast Flow (GE Healthcare, Sweden). The column (with 5 ml of stationary phase) was equilibrated with five column volumes of sodium acetate/acetic acid 10mM pH5 and 10 ml of protein extract containing bacteriocin were injected into this. After washing the column with sodium acetate/acetic acid 10 mM pH5 (Buffer A), elution was performed using a linear gradient from sodium acetate/acetic…

amino acid breakdown and will use the energy from deaminating amino acids. Phenylalanine becomes phenylpyruvic acid and is detected by acetic iron chloride, which turned the plate green when added. If the bacteria have the phenylalanine deaminase enzyme, the bacteria will become green and considered a positive result. Although not actually done in class, the oxidase is also an important test in microbe identification. In this test, cytochrome c oxidase is used as an electron carrier and become…

acid by pancreatic enzymes prior to absorption. First, the chicken is mechanically digested in the mouth when chewed and breaks it into smaller pieces. The partially digested chicken travels to the stomach and begins digesting with the action of enzymes called pepsin (Primal Pictures). Pepsin breaks the bonds that hold the protein molecule together. Once these bonds have been broken they form a chain of amino acids that are linked together called polypeptides (Primal…

This test is performed on organisms to see if it has the enzymes to reduce the Nitrate to Nitrite. To run the test, it will require using two colorless chemicals (so make sure you are paying attention) that will either change the color of the organism by the interaction of the new chemicals with the enzymes found in the unknown organism. So, if the organism has the capability to reduce the nitrate to nitrite the solution in the test tube will turn red almost immediately and the organism is…

The lab that is being preformed is about digestive enzymes. Enzymes are protein molecules produced by living organisms that speed up a specific chemical reaction in the body. There are also digestive enzymes which digest our food. Our bodies make digestive enzymes in the pancreas, stomach, small intestine, and mouth. Digestive enzymes break down the food we eat into nutrients so that our bodies can absorb them. There are different digestive enzymes that digest certain macromolecules such as…

from the cells, subsequently followed by excretion into bile or urine1. The metabolic enzymes in charge of this process are traditionally organized into two separate categories; phase I (oxidation, reduction, or hydrolysis) and phase II (conjugation) enzymes1,2. During phase I, polar groups are added to the molecules through oxidation, reduction, and hydrolysis, mediated generally through cytochrome p450 enzymes. Phase II…

Interpretation 1. Theoretical Lab Discussion This lab occurs due to enzyme kinetics, and the kinetics molecular theory. Enzyme kinetics is the study of chemical reactions that are catalysed by enzymes, and during the experiments, the reaction rate was measured by the effects of the varying conditions the substrate was in. Influence of Catalase Concentration The higher the concentration of the enzyme catalase, the function of the enzyme occurs better. As the number of disks increased, the…

syrup since it is much more effective. Pineapple contains an enzyme called Bromelain which is effective in fighting infections and bacteria. This enzyme makes pineapple juice a powerful anti-inflammatory agent. Half the amount of vitamin C that you need per day Only one glass of pineapple juice will provide your body with half of the daily dose of vitamin C that you need. Vitamin C also enables your organism to metabolize the enzymes used to ménage energy and regulate the function of the…