Restriction enzyme

The general aim of the study was to use Pymol to analyse molecular graphics of the isoenzymes carbonic anhydrase (CA) I and II, CA substrates (e.g. bicarbonate), and CA selective inhibitors (e.g., sulfonamides) to determine the relationship between CA structure and function. The specific aim of the study, described in part (ii), was to use Pymol to analyse molecular graphics of CA-I, and II, to determine relationship between structure and function and then use the results to analyse how one of…

This lab looked at the different effects of an enzyme with concentration, pH, and temperature compared to the rate at which the enzyme works. The results showed a quick decrease when the temperature and pH has passed its most favorable conditions and or levels, but the concentration remains at the same levels throughout except pH6 and 40 degrees celsius. Both the pH and temperature had a steady increase in rate once the favorable conditions were being reached or are reached, but had a certain…

Activity #1 1. Potassium and Sodium. Potassium is more concentrated inside the cell and sodium is more concentrated outside the cell. 2. Resting membrane potential is the difference in voltage of the fluids inside a cell and outside a cell. It is measured by the difference in voltage inside and outside of the cell. 3. On the outside of the resting membrane potential it would remain at 0, however on the inside it dropped the voltage from -70 to -40. This happened because with the increase of K+,…

The lab we conducted involved a process called the Maillard Reaction. When it comes to cooking meats, heating the food causes the proteins, especially those nearest the surface of the meat, to denature. These denatured proteins become more chemically reactive to other molecules in their environment. One of the primary reactions that occur when browning meats and many other foods is called the Maillard Reaction. The Maillard Reaction is a non-enzymatic chemical reaction between amino acids and…

a reaction with a catalyst. A catalyst is a molecule that starts a chemical reaction, but it is not part of the reaction it is causing. The experimenters will use pieces cut up potato as an organic catalyst. The potato contain enzymes that will perform the reactions. Enzymes, “are proteins, produced by living cells; they catalyze chemical reactions by lowering the activation energy. Activation energy is the energy required for a chemical reaction to occur” (Carter 1). Substrates or reactants are…

INTRODUCTION: What is DNA? DNA is a deoxyribonucleic acid, which is a self-replicating material that is present in just about all living organisms as the top chromosomes. DNA Isolation is the development of purification of DNA from a sampling applying a mixture of physical and chemical methods. Isolation is a routine process in molecular biology or in molecular analyses. Materials: • Electrophoresis chamber • Gel casting trays • Sample combs • Ethidium bromide • Loading buffer •…

The egg is an example of an animal cell that is placed in environments of different tonicity. The vinegar was used to dissolve the shell so that we can see the effects when we placed the egg either in a hypertonic or hypotonic solution. Since the egg membrane is semipermeable similar to the cell membrane, it only allows specific substances to pass in and out of the egg membrane down its concentration gradient. In this case, we are trying to measure to the diffusion of water that goes in and out…

In conclusion, the purpose of The Hot box device is to make it so it can use energy from from the sun to allow the Cocina del Sol Company to bake their specialty cookies while staying eco friendly. My product used aluminum foil and black paper to attract heat to my device and what’s inside of it. We heated up the box by putting it in contact with light. You can do this by letting it sit in the sun or using a lamp or anything that gives off light like one. The results from my experiments…

Abstract: An enzyme, a protein, is a catalyst, which increases the rate of the chemical reaction. The results of this experiment showed how different temperatures could affect the chemical reaction between the enzyme and the hydrogen peroxide. The temperatures tested in this lab were 30, 35,40,45 Celsius, which are close to the average body temperature which is 37 Celsius. Catalase is an enzyme which breaks down hydrogen peroxide into oxygen and water. Different temperatures were measured to see…

When conducting the different tests and streaking, aseptic techniques were followed. For every T-streaked that was done, the inoculating loop was first flame for 5 seconds and was allowed to cool for 30 seconds. Afterwards the tube containing the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty…