Restriction enzyme

was to observe the enzyme catalysis of Horseradish Peroxidase with different determining factors. In this experiment the horseradish peroxidase enzyme should have an optimal temperature of 35°C, should have an optimal pH of 6, and should catalyze rapidly with the substrate and enzyme concentration. We were able to observe that the enzyme reacts best with its optimal temperature of 35° C and optimal pH of 6. Moreover, when there’s a high the substrate concentration and a high enzyme concentration…

IRRADIATION Food irradiation is the processing of food products by ionising radiation in order to control food borne pathogens, reduce microbial load and insect infestation, inhibit the germination of root crops, and extend the durable life of perishable produce (International Consultative Group, 1991). Application of ionizing radiation results in the radiolysis of water. This triggers the development of species such as OH−, hydrated electron and H+, which can then induce several chemical…

There are two major classifications of living things, plants and animals, and all species that are under these two kingdoms are required to have proper functions of their body processes to survive. The most important in all of the body processes is the transport system, it is the one that is responsible for well being of all other body systems to function smoothly and properly by supplying nutrients for it to live.These organisms need to have the ability to transport particles such as molecules…

Methods 1. Treatment of barley with endophyte. • Five types of Barley were treated with the endophyte Pseudomonas fluorescens L321. • They were innoculated with the seed coating mixture ( 4% sodium alginate and 1% skimmed milk powder) without the bacteria. The bacteria were grown up overnight in LB broth supplemented with 3% trehalose (400ml per strain) , centrifuged and then added and then resuspended in the coating mixture. • 30ml of inoculant mixture was then added to 1kg of seed in a…

Some physical and chemical properties of watermelon rind and sharlyn melon peel powders Proximate composition WAC, OAC and antioxidant activity of watermelon rind and sharlyn melon peel powders Data in Table 2 showed that the watermelon rind powder had higher moisture, ash, fat, protein and carbohydrates (10.61%, 13.09%, 2.44%, 11.17% and 56.02%, respectively) as compared to sharlyn melon peel powder. On the other hand, the sharlyn melon peels had higher content of crud fiber (29.59%) as…

5. Protein purification There are many thousand kinds of proteins with different properties and functions in a cell. Proteins have been purified in active form on the basis of such characteristics as solubility, size, charge and specific binding affinity. Normally, protein mixtures are subjected to a series of separations, each based on a different property to yield a pure protein. 5.1 Separation based on solubility (Scopes, 1994) The protein solubility in various solvents depended on the…

In 1935, the first plasma membrane structure model was introduced by Danielli and Davson. They proposed that the membrane is made of phospholipid bilayer that consists of hydrophobic head and the hydrophilic tail. Hydrophilic head, which is polar, facing towards the water molecules and said to be coated with protein. Hydrophilic tail, which is non-polar is not facing water molecules and sandwiched between the two bilayers. At regular intervals of the surface of the plasma membrane, there are…

The effect of CKD on sludge stabilization compared with chemical stabilization with Lime is introduced in Table (3). Also comparison between different concentrations of sludge is introduced. Different dosages of CKD were examined and the dosage that achieves stabilization was 50% and 60% for thickened and diluted sludge respectively, while the required dosage of Lime was ranged between 22 and 29%. The required lime dosage complies with the results of EPA [4], Wang [18], Rahman [11] and Salah…

the rate of reaction on Enzyme reaction Introduction: This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalase. The biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed. Enzymes are globular proteins folded into a complex 3-dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically. Enzymes speed up reactions…

In this essay, I will explain how we can benefit from bonding of atoms of certain elements in our daily life. As I will show in this essay, with the help of understanding the surface chemistry, which is the study of chemical reactions at interfaces (surfaces), different desired affects can be produced by the formation of selected elements such as making improved chemical composition of a surface. Surface chemistry today can be applied to solve various issues and problems including fighting…