Restriction enzyme

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    needed to start a chemical reaction, activation energy, comes from enzymes. Enzymes (almost always proteins) are organic catalysts that help speed up the rate of reactions and allow the reactants to get the most out of the energy available. They do this without changing the reaction. Without an enzyme, then many chemical reactions may not occur because the activation energy could be too high to be reached without the help of an enzyme. For example, table salt, or sucrose, could sit for years…

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    P-Nitrophenol Lab Report

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    Chapter 4 - Discussion A standard curve of p-nitrophenol was drawn at the start of the project, before any enzyme assay of the fractions. The standard curve is set up so the equation of the line can be used to measure the enzyme activity in each sample fraction. P-nitrophenol shows a linear relationship with acid phosphatase. An Enzyme assay gives the activity levels of acid phosphatase in each fraction. The results from each fraction were placed into the equation of the line from the standard…

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    Yeast Fermentation Report

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    Jenna Bach Marula Wine: Marula Sunrise Organic Chemistry Investigation Table of Contents Introduction Hypothesis Report Method Apparatus Tabulation of data Graph Analysis of results Evaluation of Investigative Procedure Conclusion Bibliography and Referencing Introduction The fermentation of the fruit can occur naturally by crushing up the fruit and leaving the skins in the mix for a day as the skin has natural yeast which can aid in the fermentation process. The…

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    Phytic Acid In Yeast

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    RESULTS Phytic Acid The concentration of phytic acid was determined spectrophotometrically in three tested plant materials of Gishta as shown in table 2. The concentrations of phytic acid were obtained from 5 g dried leafy material. All the leaf samples contained phytic acid (detection limit 0.025 mg/ml) in this study. This may have been due to the fact that there was a limitation in terms of the detection concentration since concentrations less than mg was not tested. Seed extracts was found…

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    Cow Liver Research Paper

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    Background Information: Enzymes are catalysts for chemical reactions in living things, they help the body speed up the rate of virtually all of the chemical reactions that take place within cells.They are very important, enzymes are biological catalysts. Enzymes don’t need a lot of energy to start a chemical reaction, it all depends on the body's temperature. Enzymes need catalysts, catalyst is a substance that decreases the activation energy needed to start a chemical reaction. Enzymes are…

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    Catalytic Pocket Essay

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    Result and Discussions 3.1. Changes in catalytic pocket Instead of having the conventional catalytic triad residues (His665, His667 and Asp767) in it’s protease domain, Lon protease employs the Ser679 – Lys722 catalytic dyad to form a catalytic pocket for promoting proteolysis. But the mutations in catalytic dyad residues make some changes in the catalytic pocket of these three mutant types. A comparison of SASA values and RSA values [Supplementary file 1] were done among the catalytic…

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    The Citric Acid Cycle

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    Abstract The citric acid cycle, also known as the Krebs cycle or tricarboxylic acid cycle, is central to the formation of usable energy forms in cells. This essay will give detail on how this is achieved and the cycle’s links to other metabolic pathways such as oxidative phosphorylation. It will also explore how the cycle functions in anabolic and catabolic forms while replenishing used intermediates before examining how the cycle is regulated. Introduction The citric acid cycle being named…

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    Animalia Research Paper

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    "Animalia" redirects here. For other uses, see Animalia (disambiguation). Animals Temporal range: Cryogenian – Present, 670 –0Ma Had'nArcheanProterozoicPha. Animal diversity.png About this image Scientific classification e Domain: Eukaryota (unranked): Unikonta (unranked): Opisthokonta (unranked): Holozoa (unranked): Filozoa Kingdom: Animalia Linnaeus, 1758 Phyla List of animal phyla Synonyms Metazoa Animals are multicellular eukaryotic organisms that form the biological kingdom Animalia. With…

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    Objective: In-silico assessment analysis of Protein database entries of the Pf. lactate dehydrogenase (PfLDH) enzyme, considered as an important target for designing of anti-malarial drugs. Methods: PDB structures of the enzyme Pf. lactate dehydrogenase were downloaded from Protein Data Bank. These were subjected to ANOLEA energy assessment analysis and Swiss model was used to analyse Ramachandran plot. Results: The energy assessment analysis and analysis of Ramachandran plot were carried out…

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    Chitocan Case Study

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    Preparation of coating solutions Chitosan solution was prepared with 2 % (w/v) chitosan in 1% (v/v) acetic acid glacial. The solution was placed on a hot plate/magnetic stirrer for 6 h. The resultant chitosan-based coating solution was filtrated, through a Whatman filter paper No: 2, glycerol was added at 0.75 % (w/v), and stirred for more than 15 minutes. Two of the best concentrations, 60 and 90 mg mL−1 were prepared from freeze-dried IgY for S. putrefaciens and P. fluorescens respectively.…

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