Purpose:The purpose of this experiment was to use fermentation to produce ethanol from sucrose.Procedure:See handout for procedureResults:Table 1. Balanced Equation for the Conversion of Sucrose into Ethanol퐶퐻푂 + 퐻푂 ⟶ 4퐶퐻푂 + 4퐶푂퐶퐻푂퐶퐻푂12푋12 = 1442푋2 = 2422푋1 = 22 6푋1 = 611푋16 = 176 1푋16 = 16144 + 22 + 176 = 342 푔/푚표푙 푠푢푐푟표푠푒24 + 6 + 16 = 46 푔/푚표푙 푒푡ℎ푎푛표푙(2.0푔 푠푢푐푟표푠푒)(Table 2. Weight of Ethanol Product and Percent Yield of Fermentation ReactionWeight of Fraction 10.309 gWeight…
on them. This indicated that there was an error because the slides should all look very similar. Since I had some background information on staining from microbiology laboratory, I started to observe that some slides were left in ethanol for longer periods of times. Ethanol is used to dehydrate the slides and that’s why some slides were over-decolorized. Research shows that there are a variety of different protocols for Cresyl Violet staining.1 The indicated time that the slides…
cervesiae are responsible for ethanol tolerance and production? Can we alter them using gTME to increase ethanol/glucose tolerance and have more efficient ethanol production? 2. Answer the following questions regarding the figures below. Answers should be 1-2 sentences/phrases at most (complete sentences not necessary). Figure 1B a. Why was this experiment done? (Purpose): Identify which gTME mutants had the highest growth rates in presence of high glucose ethanol concentrations.…
NIMOTOP INJECTION Nimodipine 0.02% solution contains the following excipients: Ethanol 96%, Macrogol 400, sodium citrate, citric acid, Water for Injections Ph. Eur. These excipients consist of properties that aim towards the proper functionality of Nimotop. They consist of inherent stabilities that cause little or no effect in the stability of the drug and they do not have any acute or chronic toxicity. The excipients are also added into the Nimodipine 0.02% solution to enhance the biological…
3-(4-acetylphenyl)-6-bromo-2-(4-chlorophenyl)quinazolin-4(3H)-one (2) Compound 1 ( 0.01 mol) fused with p-aminoacetophenone ( 0.01 mol) at 160 oC in an oil bath for 2 h. Crystallized from ethanol to give redish brown crystals of 2, m.p. 210oC in 80% yield. Analysis for C22H14BrClN2O2, Calcd.: % C, 58.24; H, 3.11; N, 6.17, Found: % C, 58.31; H, 3.17; N, 6.20, IR spectrum (KBr, cm-1) showed characteristic absorption bands at 3380 (C-H aromatic), 1710 (C=O of acetyl), at 1690 (C=O of…
copper turnings, sand bath, ring stand, a thermometer adapter, and a thermometer1. Before assemble of the distillation apparatus could begin, the 50/50 mixture of ethanol and water needed to be prepared. Using a pipette, 2 mL of water was…
fermentation can be one way to make ethanol using yeast (Bourne). Using this method of making ethanol adds oxygen to diesel/petroleum blends, which reduce unburned hydrocarbon emissions and reduces the rate of greenhouse gases. In addition, it is also better for cars since it increase engine…
petroleum-based economies to bioenergy sustained economies is of utmost importance. In the United States, corn is considered to be a cheap energy crop and is widely cultivated for ethanol production. In contrast to corn, switchgrass is an easily cultivated plant that can be used for lignocellulosic biomass and ethanol production. Switchgrass (Panicum virgatum L.) is a warm-season perennial grass. As a native to North America, this grass is thought to be significantly dominant in prairies…
demonstrator: H.Helbert Reaction Equation Summary Salicylamide and sodium iodide are dissolved in ethanol, and stirred and cooled to 0. After that household bleach was added while stirring vigorously, solution changed from colorless to pale yellow. Sodium thiosulphate and hydrochloric acid were added aswell. Product collected by vacuum filtration and recrystallized from 96% ethanol. IR- and H- NMR-spectrum were taken to determine the location of the I+. I had a yield of 0,7…
TRIS Hcl, NBT, NADH, Ethanol, PMS, l-ascorbic acid 1. The superoxide radicals is generated in 3 ml of TrisHCl buffer (16 mM, pH 8.0) containing 1 ml of NBT (50 μM), 1 ml NADH (78 μM) and ethanol extract of sample (25 – 50 μg). 2. The reaction was started by adding 1 ml of PMS solution (10 μM) to the mixture. 3. The reaction mixture was incubated at 25° C for 5 min, the absorbance was read at 560 nm using a spectrophotometer (Schimadzu UV-Vis 1700) against blank samples using l- ascorbic acid…