“DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD FOR SIMULTANEOUS ESTIMATION OF CEFTRIAXONE AND TAZOBACTAM INJECTION USING RP-UPLC METHOD”
To develop and Validate Stability Indicating Method for simultaneous estimation of Ceftriaxone and Tazobactam in their combined dosage form by RP-UPLC method in various stress condition like, alkaline, acidic, oxidative, thermal and photo degradation.
2.4.1 Chromatographic Condition
Column Separation was achieved by…
Adsorption isotherm models :
To study the proper model, the isotherm data were analyzed and were fitted them into two models, Freundlich and Langmuir, because they are the most suitable and employed models.
Equilibrium uptake of CV and MB was investigated with sorbent weight of 0.5 g of fennel seeds in contact with 100 mL of solutions and at pH 4 for CV and 6 for MB. The flasks were shaken at 200 rpm and the equilibrium concentration of the remaining CV and MB was determined by spectrometer.…
All standard purified materials were used for this study. The solvents which are used in the preparation of solutions should be HPLC grade and obtained from Merck Specialties Private Limited,Mumbai.Active pharmaceutical ingredients Decitabine were supplied by Spectrum Pharma Research Solutions, Hyderabad. The formulation was purchased in a local market. Instrumentation The High performance liquid chromatography system consists of A suitable HPLC having a isocretic system equipped with auto…
1-pyrenehexanoic acid was used as the 5-carbon linker and fluorescent component of the target molecule "PYRET-5". This molecule was purchased pure along with the sugar Trehalose, the molecule to which the pyrene was to be attached. Other reactants and solvents include HATU, HBTU, N, N-Diisopropylethylamine (DIPEA), sodium azide, Dimethylformamide (DMF), and Diphenyl phosphoryl chloride and were bought pure or used as received. All reactions were run at room temperature (298.15 K) and standard…
ENVM COMPREHENSIVE EXAM
NAME: Sheetal M Patel
STUDENT NUMBER: 540249
ENVM 665 Risk Management: Question 1
What are the two major general types of chemical sources that result in human exposure to those chemicals and provide a simplified “total” human exposure model showing the pathways that determine the total human dose from those chemicals.
The total Human exposure model
6. The first step in metabolism of ethanol in humans is the oxidation of ethanol to acetyldehyde by the enzyme alcohol dehydrogenase (ADH). At 298 K and pH=7.0, ethanol is incubated with ADH and the concentration of ethanol and acetyldehydee is measured over time.
a. Using the results above, calculate the standard free energy change (ΔGº’) for this reaction. Explain your calculations.
The standard free energy change can be expressed through the formula ΔGº’=-R(gas constant) T(temp in Kelvin)…
Preparing Dilutions of the Silver Nanoparticles
1. Gather all materials.
2. Put on a pair of disposable gloves.
3. Make a paper towel damp with some 70% isopropyl rubbing alcohol. Wipe down the work surface with the rubbing alcohol-dampened paper towel.
4. Using a permanent marker, label the five baby food jars #1 to #5.
5. Using the 25 milliliter (mL) graduated cylinder, measure and pour 22.5 mL of distilled water (from a new, unopened container) into each of jars #2, #3, #4, and #5.
Experiment 2. Steam Distillation
The Isolation of the natural product trans-cinnamaldehyde from Cinnamon bark
This experiment aims at separating cinnamaldehyde from the cinnamon bark. A mixture of volatile and non-volatile elements is separated through the steam distillation method with temperature being below 100 oC. Moreover, the method is used to extract essential oils. The cinnamaldehyde will be conveyed up with the distillate, observed as an emulsion on the condensed vapor. It…
In Experiment 12B we used an umpolung reagent in an umpolung synthesis to synthesize benzoin from benzaldehyde. Umpolung synthesis is a synthetic approach that allows us to switch the identity of a reacting species. For example, the normal chemical reactivity of a nucleophile can be switched to one of an electrophilic compound. Two common agents are cyanide and thiamine (Vitamin B). The mechanisms for both agents are the same. In this experiment, thiamine was used to turn benzaldehyde, an…
reduces pain and inflammation by limiting certain hormones. The drug is taken orally, usually in the form of a pill, and is absorbed into the bloodstream once it dissolves. Both human saliva and human stomach acid aid in dissolving the pill. Human saliva has a pH of about 6, and stomach acid has a pH of approximately 2. The purpose of this investigation is to discover whether the pH of a solution will affect the time needed to dissolve the ibuprofen.
If the pH of a liquid is…