Silica gel

The objective of the experiment was to separate chlorophyll a and chlorophyll b and the alpha and beta conformations using the paper and column chromatography. The two techniques that were used to separate and identify the plant pigments were the paper chromatography and column chromatography. For the mixture to be separated, it must first be applied to a stationary phase. The stationary phase in the paper chromatography experiment was the filter paper. The mobile phase used in this experiment…

small amount of cotton. An Erlenmeyer flask was placed underneath the column to collect any excess material. Eleven 20 ml vials were labeled numbers 1 through 11. Silica gel was measured to 42 ml using a graduated cylinder and then moved into a 150 ml beaker. A slurry was created by adding 50 ml of hexane to the beaker containing the silica gel. The slurry was stirred for about one minute. This was the stationary phase for the column chromatography. Pour the slurry into the column that is…

left in the solution that was not dried out by the ethanol. [1] Water will move toward the more hypertonic solution and separate from the DCM extract. After successful separation of the pigments from the spinach leaves, the pigments are put into silica gel and the solvent is allowed to evaporate off so that the pigments (now in powder form) can be put through column chromatography to separate the chlorophyll and β-carotene pigments. Column chromatography is a means to divide chemical compounds…

amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its polar properties. This makes it easier to drive the eluent away from its binding area. An eluent is a liquid that…

of suspended silica gel was poured into the column using a glass powder funnel. An addition 33.0 mL of 3% ethanol in DCM was added to the column to rinse out the Erlenmeyer flask and powder funnel. The solvent was pipetted into the column in a circular motion to get the remaining silica gel off of the walls of the column. The stopcock of the column was opened and the solvent was collected in another Erlenmeyer flask until the solvent level in the column was 1 cm above the silica gel. The…

Firstly, 60 mL of silica was added to a 250 mL beaker. After which, an approximate 100 mL of chloroform was added to the beaker to make a pourable slurry. The slurry was then poured into the column and the solvent just about the silica was drained off into a conical flask. To minimize losses, the silica slurry beaker was rinsed with more chloroform and added to the column. Next, a thin layer of acid washed sand (3mm – 4mm) was added to the column. Next, two ‘curcumin soft gels’ was puncture…

Current Chromatography. In TLC, a slide of glass coated in a substance can act as the stationary phase, serving as a bed for the mobile phase to move along. Samples are applied at a certain height to be carried and separated. Using this method, a silica gel coated aluminum plate is placed in the confines of a glass jar. At the bottom of the jar lies the liquid solvent (mobile phase). The process is usually started off by marking the height of…

interaction with the adsorbent and stay near the top and move down at a slower rate. The less polar substance has a weaker interaction and is able to separate and move down the column at a faster rate. These components are seen as colorized bands. The silica gel allows for a greater separation as it acts similarly to the theoretical plates found in distillation, with different equilibria occurring from interactions between the components of the mixture and its own structure. Thus, the eluted…

molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram negative rod shaped marine bacteria that exhibits bioluminescence and is both found free-living or in symbiosis. It has a variety of hosts such as the bobtail squid and is named after the German scientist…

abundant element, and it can’t be found free in nature. Silicon is usually found as silica or silicates. In order to obtain silicon by itself sand or carbon needs to be heated. In the next paragraphs, I will be discussing the physical appearance of silicon, chemical formula, molar mass, and Lewis structure. Jons Jacob Berzelius Swedish chemist first discovered silicon, he made the discovery by heating up potassium and silica in which then isolated silicon. Silicon natural occurring form is…