Silica gel

this lab activity, the experiment was divided into part one: DNA Purification from Buccal Swabs, part two: PCR (polymerase chain reaction) amplification of the d1s80 VTNR locus from human genomic DNA and Part three: Analysis of PCR products using gel electrophoresis. We had a specific activity to carry out during each lab section. PART ONE: DNA Purification from Buccal Swabs The materials used for this lab section are listed below: Materials • Sterile…

To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because…

the immediate success of their hair product prompted them to expand their vision. What they discovered was that some of the hair styling products were too greasy for their customers tastes, and others just didn’t get the job done. It has a thick gel like consistency that makes it easier to apply. Three of the ingredients help to formulate the soft texture of this pomade. Parfum, Coumarin and Lanolin give user’s hair a soft smooth feel with a firm hold. The ingredients give it an agreeable…

In this experiment Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was performed under reducing conditions to estimate the molecular weight of proteins within a sample. The protein sample was from the previous using anionic exchange chromatography to partially purify N-acetyl--D-hexosaminidase, Lab 3. The molecular weight was determined through the interpolation of a standard curve of proteins with a known molecular weight. The molecular weight of band 1 was determined to…

was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small molecules is greater than that of large…

Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard. Methodology: The experiment begins with the denaturing of the proteins through heating by…

column leaving just the DNA in its membrane. Later some Elution Buffer was used to separate the DNA from the membrane of the spin column. Day two consisted of making the plasmid DNA resistant to Kanamycin and Ampicillin. Day three we made 0.8% agarose gel and ran electrophoresis to be able to see the weight of the DNA. Day four we genetically transferred the ligated plasmid DNA to E.coli and plated the cell mixture. Day five we looked at our results and went over them. All of this works together…

Agarose Gel Electrophoresis Since the discovery of the four classes of macromolecules (proteins, lipids, carbohydrates, and nucleic acids), researchers and biochemists have fine-tuned methods that reveal structural information about each of them. Different structural properties determine different functions and chemical properties, and scientists have developed intricate techniques to observe, quantify, and isolate molecules based on their aforementioned properties. Agarose gel electrophoresis,…

Article Comparisons to Southern (1975) Article One The article “Detection of specific sequences among DNA fragments separated by gel electrophoresis” (1975) by E.M. Southern focuses on the way that the fragments of DNA can be transferred from agarose based gels to cellulose nitrate filters. Then fragments themselves are hybridized to active RNA. E.M. Southern’s main influences for the investigation were the studies of Smith and Wilcox (1970), and Kelly and Smith (1970), which showed that the…

them every so often and you do have to occasionally remember to check the batteries, some need batteries ever 4-6 months. If you forget, you could end up with mold or mildew despite your best efforts. In some battery powered dehumidifiers, they use silica gel to ensure that everything stays dry. The biggest type that most people avoid because of its inconvenience is the desiccant dehumidifiers. Depending on the type that you use, you may have to deal with extreme moisture that drips before you…