Silica gel

Sweta Soni 2/22/15 Biochem Lab Experiment 5 Introduction The purpose of Experiment 5-1 is to separate a mixture of three molecules—blue dextran, cytochrome c, and potassium chromate—through the use of gel-filtration chromatography. The volume at which each molecule elutes will be recorded in order to determine the accuracy of separation. The purpose of Experiment 5-2 is to purify the E. coli enzyme, β-Galactosidase through the use of affinity chromatography on…

Discussion When comparing the banding patterns of the crime scene to those of the suspects, the resulting gel indicates that Suspect 2 was at the scene of the crime. Although enzyme 1 produced identical DNA fragments across the gel, enzyme 2 did not. This is evident in lane D and possibly indicates that this enzyme was unable to bind to recognition sites similar to the crime scene DNA in well B. Thus, it produced a DNA fragment smaller in size that travelled further. Since the DNA evidence in…

they weigh down hair or make hair have a slimy texture. (Reviewer, Walmart, March. 14, 2015) The goal is healthy hair, not masked hair. There are more than just hair masks that help damaged hair. These brands also have deep conditioners, shampoos, gels, mousses. and sprays that are dedicated to damaged hair. There are also attempted solutions that don’t work. Most of these for sale “solutions” are store brand products. For instance Wal-Mart, Kroger, and Target all have products that have been…

DNA to come inside and interact with the bacterial DNA. The DNA gel electrophoresis showed that there were no fragments…

In this experiment, a SDS-PAGE gel was used to analyze the protein samples from the MBP-AP and WT-AP experiments. The samples are then referenced to the ladder to determine the molecular weight of the MBP-AP and WT-AP proteins. Then the UV absorbance of the two proteins from 240 nm to 340 nm is determined using a nanovolume cuvette. The absorbance at 280 nm was then used in conjunction with data from previous experiments to determine the concentration of the MBP-AP and WT-AP protein samples.…

using agarose gel electrophoresis. Species identification was performed by importing finalized CO1 sequences into two DNA databases to ensure accurate identification. Phylogenic analysis was then conducted to determine relationships between identified species. Electrophoresis Electrophoresis of DNA run in 2% agarose gel showed successful extraction of DNA in samples 1-8, 12 and 14-16. Samples were of good quality with high molecular weights (Figure 2). Samples 11 and 13 in Row 1 of gel 2 were…

the DNA from A17 and was stored at -20 oC until gel electrophoresis. Gel Electrophoresis To determine the success of DNA extraction and later PCR clean-up the samples went through gel electrophoresis. DNA extraction was tested by putting the samples in the loading chambers of the supporting matrix which was made of one percent agarose gel. GelRed, a nucleic acid dye, was added to help visually identify the DNA bands. A power supply was added to the gel for 20 minute by placing an anode…

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…

hair products, mostly used by both men and women are hair gel, hair cream, hair mousse, hair serum and hair spray. The gel is used to solidify an area of hair to create a specified style. Some negative effects of the use of hair gel is hair loss, dandruff, discoloration and damage to the hair and dehydrating of the hair (Goenka, 2013). Hair cream is similar to hair gel because it also hardens and styles the hair, but in cream form rather than gel (Cioffi, 2010). Using an overload amount of this…

As DNA is a negatively charged molecule the samples when run through the gel move towards the positive end of the gel, this is important to remember during the setup of the gel so that the electrodes are placed correctly. For each region of DNA under investigation the size of the molecule depends on the amount of nucleotides within that sequence, for the first experiment this…