Gel electrophoresis

In Procedure 6.1, two different gels were used. One gel was 2.0% agarose while the other gel was 0.8% agarose. The reason we poured two gels was because each gel had a different purpose. The 2.0% agarose (thicker gel) was used to determine amplicons while the 0.8% agarose (thinner gel) was used for restriction fragments. One gel was ‘thicker’ than the other gel to distinguish smaller segments than the thinner gel. By having a thicker gel, smaller segments can move better and not be cleared out…

Figure legend: Figure 3 shows the graphed bands given from the picture taken from the blue box after electrophoresis. The graph provided an equation that then allows to solve for the other established base pairs. Figure 4 Figure legend. Figure 4 shows the restriction map of the plasmid indicating the restriction sites. Discussion The gel was analyzed through the method of agarose gel electrophoresis. Once the process was done, a picture was taken to show the distances between the bands as…

Introduction In the United States a suspect in a crime is innocent until they are proven in court of law to be guilty beyond doubt(Cornell web), for now at least. This need to prove guilt in a suspect means the use of DNA to determine if the suspect was at least present at the crime scene is a powerful tool because no one person can change their DNA at will. The use of DNA as way to identify people has it 's origins in the United Kingdom because of a technique created by Dr. Alex Jeffreys used…

damages. Students must keep hands away from face, eyes, and mouth, when being in contact with chemicals. Students will enter this lab with basic knowledge on how agarose gel electrophoresis is used to separate proteins. This lab experiment will approximately take 20 minutes to gather all the materials needed and to prepare the agarose gel for use. The run time for the experiment can range between 2 to 3 hours depending on the accuracy and pace of the students. It is important to follow…

function of gel electrophoresis? Way to sort and measure DNA strands according to length. This technique is also useful for separating other types of molecules, like proteins. 2. Where are DNA samples placed in the gel? DNA samples are placed into wells at one end of the gel. 3. What makes the DNA move across the gel?
By adding electrical current DNA moves. 4. Why do DNA fragments of different lengths travel to different locations on the gel? **Short fragments move through the wells in the gel…

experimental restriction enzyme since they believed it would make the mutant DNA linear since it would cut the plasmid once while leaving the wild-type DNA uncut and supercoiled. This would make the wild-type DNA travel faster through the agarose gel in gel electrophoresis. They also chose a positive control group with the HindIII restriction enzyme that would cut both the mutant 1 and the wild type plasmid once and at the same position. Then they had a wild type and mutant 1 plasmids that had…

sample was separated through gel electrophoresis. This project entails the interpretation of fluorescing bands from gel electrophoresis in order to determine the number of tandem repeats present on an individual’s alleles. The total number of tandem repeats is expected to fall between the experimentally observed range of 21 and 33 repeats [4]. The results show that the individual’s D1S80 genotype was successfully calculated through measurement of the gel electrophoresis bands and this subject’s…

and this experiment illustrated the versatility of these molecular biology techniques of PCR and gel electrophoresis to serve as methods identifying individuals. Both of the hypotheses made in this experiment that (1) there were genetic variation with the six students and (2) Student 3’s genotype was heterozygous were supported by the data. As seen in Figures 1 and 2 and Table 1 with different agarose gel percentages, it can be concluded that each of the six students are genetically variable and…

what had caused the tumor. We will be immunoblotting (western blot) for the ATM protein (3). The tumors will be isolated and grown in culture so we have enough samples to work with (1). We prepare the samples for two-dimensional gel electrophoresis using 1D/2D Electrophoresis ZOOM IPGRunner System[1,3]. This process allows the proteins to be separated and particularly were looking for the ATM protein (1). The proteins are separated by their natural charges and dissolved in a solution of…

Identifying an Unknown Plasmid Through the Process of Gel Electrophoresis Introduction: Biotechnology requires certain techniques and methods that help identify plasmids, which can be used for forensics, DNA fingerprinting, etc. In this class, each lab focused on teaching the process of using the correct techniques used to identify a plasmid. Plasmids are pieces of DNA that are circular and relatively smaller than chromosomes. They aren’t important in the sense that they don’t carry out…