Gel electrophoresis

sample without any enzymes; finally, Polymerase Chain Reaction (PCR) was used to amplify purified DNA. Overall, in the ligations, miniprep, restriction digest reactions, and PCR experiments, every sample was analyzed and identified by using gel electrophoresis. In one experiment, transformations of E.coli were plated on the wide variety of petri dishes and left to be incubated overnight in order to observe green fluorescent bacteria colonies over using the UV…

1-13 nucleotides long that are repeated several times in tandem array.In DNA profiling, the alleles of a selected number of different STRs are determined(Brown, 2006).When restriction enzymes are used to cut STRs and the results areanalysed (by electrophoresis or southern blotting a technique not investigated in thisexperiement), a pattern of bands are produced which is described as a ‘DNAfingerprint’ as it is unique for each individual (Cummings, 2003).DNA-modifying enzymes that cut DNA at…

recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed using FIGE gel Electrophoresis. The data that was acquired consisted of 12 red colonies and 483 white colonies from the conjugation mixture with a conjugation efficiency of 2.42%. The FIGE gel Electrophoresis produced the DNA bands of the donor and transconjugant strains with a size of 112 kb and no bands for the recipient. The results show that the…

cognitive disorder affecting more than 28 million women in the US alone (Migraine Research Foundation, 2016). To find SNPs, the control and mitochondrial region of DNA was isolated, followed by Polymerase Chain Reaction (PCR) amplification and gel electrophoresis to see the isolated DNA. For the PCR segment, base pair (bp) approximations of 500 (Figure 1) and 1100 were found for PCR Products A and B respectively. RFLP did not result in significant sites which provide suitable evidence of SNPs.…

lover of being the biological father. Unfortunately, blood typing was inconclusive as both men have the same blood type. So the judge ordered DNA analysis. A sample of blood was taken from the mother, the baby, and both men. The results of the gel are shown below. Who is the father of the baby? Which bands (1-11) are most useful for determining the father of the baby? Why? The lover is the father of the baby. The bands most useful to determine the father of the baby are bands 4 and 8.…

A plasmid is circular, relatively small in size, double stranded as a DNA molecule, and is physically distinct from the chromosomal DNA of a cell. Innately found in prokaryotic bacterial cells, plasmids can sometimes be found in eukaryotes as well 1. Plasmids are self-replicating; therefore, when a plasmid is formed, infinite copies of the plasmid can be created if the plasmid is grown in bacteria. 2 Because of the size of plasmids, which can range anywhere from one thousand base pairs up to…

the natural replication process of DNA and complementary nucleic acid hybridization. The goal of the PCR is to produce required amount of the target DNA region that is be analyzed. PCR amplified DNA is sent for sequencing and visualized by gel electrophoresis. It also can be cloned into plasmids. PCR is used in many fields of medicine, molecular biology research, medical diagnostics, and ecology. It is fast and inexpensive technique used to amplify DNA and RNA fragments by 107 times. The…

The science of my topic is revolves around criminal law and forensic science. According to staffs. ac. uk “Forensic science is any science used for the purposes of law, and therefore provides important scientific evidence for use in the courts of law, e.g. in a criminal investigation and trial.” Today's world got a lot of new technology and that makes it easy for forensic scientists to solve more crimes than ever before. Also the field has grown exponentially over the years. Forensic science…

Electrophoresis will be carried out in basic medium, composed of a TBE IX buffer (0,89M Tris, 0.8M Boique acid, 0.02 mM EDTA; pH 8.4 disodium). The nucleic acids, polyanionic macromolecules uniformly negativemen loads, when placed in a constant electric field will then migrate through the gel towards the anode. The speed of movement of the fragments through the gel mesh will therefore vary depending on the concetration in agarose gel, but also the molecular weight (number…

10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution to antibiotic resistance because phages are viruses that are able to infect bacteria. This can tremendously help and set a new pathway in biology. The results were that phage was isolated. The conclusion is that gordonia host didn’t produce results, which…