Gel electrophoresis

charge of the protein with a negative charge. By using SDS the protein involved in this experiment allowed the different volumes of the protein to migrate down the electrophoresis gel at the same time. SDS is also present throughout the gel to make sure the proteins are linearized and their charges are masked throughout the run. The gel run is conducted…

PCR machine to amplify DNA samples. Once the PCR cycle was complete, samples were stored in a freezer at -20⁰C. With the products from PCR, students used Gel Electrophoresis to separate electrically charged molecules. Gel Electrophoresis requires 3 steps; preparing a gel solution, gel electrophoresis, and photographing the gel. To prepare the gel, students mixed .3g of agarose and 30mLs of 1X TAE buffer in a 125 ml flask (Penn State Biology 220W Lab Manual, pgs.55-59). The mixture was microwaved…

agarose gel electrophoresis was used in to analysis the DNA that was extracted. The nucleic acid is at PH of 8 which allows the material to move down the electric field. The nucleic acid moves from positive to negative electric field. The ETBR is used to make the nucleic acid visible in the fluorescent light and the binding to the DNA. The “stop buffer” which is EDTA and bromophenol blue. The Bromophenol helped make the progress of making the DNA visible when it is injected in the gel and the…

examined the other two loci, but gained limited data from them. To analyze the seal samples, the researchers extracted DNA from the samples and amplified the short tandem repeats using PCR. Then, the scientists ran the PCR products through agarose gel electrophoresis stained with ethidium bromide. The researchers measured the sizes of the bands by comparing them to standard fragments with known sizes. After the completion of the process, the scientists compared the seal’s genetic similarity by…

Scientific experiments and observations have led to many discoveries, which in turn have allowed scientists to develop a better understanding on the way diseases originate in humans. Molecular biology in particular has allowed scientists to discover the way in which domains, found in proteins impact a genome. Domains are autonomous folding units that carry out specific functions and mechanisms in a certain protein. These functions consist of being responsible for interactions in the cell, which…

Materials 1. Micropipette 2. Micropipette Tips 3. WARDS’S quick view DNA Stain 4. Prepared Agarose 0.8% 5. Tris-Borate-EDTA 5x Buffer 6. Electrophoresis Mold 7. Electrophoresis comb 8. Electrophoresis chamber 9. Electrophoresis power pack and cords 10. DNA from cow liver 11. DNA from chicken liver 12. 224 grams of fresh cow’s liver 13. 224 grams of fresh chicken liver 14. Meat tenderizer 15. Kitchen knife 16. Blender 17. 2.5 ml Salt 18. 31 ml dishwashing liquid (Dawn) 19. 354 ml warm water 20.…

should have bacterial lawn as results showed. With better and more accurate results, the transformation efficiencies would have changed as well with higher numbers in ug units. Miniprep To Purify And Isolate plasmid DNA-  Figure 3: Miniprep Gel Electrophoresis. Figure 3 Key: Components of each well. The photo shows the results of the Miniprep, which allows for the identification of the unknown sample. Well #3 (the unknown) has the highest band above the 10kb line, another band a…

regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins can be analyzed using SDS-PAGE (sodium dodecyl sulfate Polyacrylamide Gel). This technique is useful since it denatures and coats each protein being tested with the same charge, using β- mercaptoethanol and DTT. Gel- electrophoresis is then run to compare the sizes of different proteins. The goal of this experiment was to learn about the process of SDS-PAGE and also about gene expression by…

The crude extract containing a catalase, catalyse a reaction where the substance is simultaneously oxidized and reduced, giving two different products of H2O to O2 and H2O. This reaction was measured by UV-Vis spectroscopy which results a decrease in absorbance at 240nm. The decrease in absorbance was caused by the loss of H202 in a product. The catalase enzyme was purified by the 5 – step procedure using a sodium phosphate buffer and a DEAE- Sepharose column which is mainly used for protein…

Purifying DNA to Estimate its Purity using PCR Amplification of VNTR to Load and Run Agarose Gel Introduction The study of this experiment was the Dopamine transporter gene. This gene is associated with different brain disorders like bipolar, as well as certain behavioural traits such as ADHD.[1] Dopamine transporter gene is a presynaptic plasma protein containing different VNTRs in it’s UTR and plays an important role in restricting the activity of dopamine by rapid reuptake into the…