Catalysis

The objective of this lab was to see how enzymes were used, use the scientific method, and how enzymes are affected by temperature. Introduction Proteins which constitute enzymes in their final form are macromolecules that make up large amounts of living organism which are called polymers which are made up of many different monomers. An enzyme is a biological catalyst that increases the rate of a reaction by lowering activation energy. Activation energy that the enzyme uses id an energy barrier…

There are thousands of diverse amylase and each has different optimal temperatures in which they complete their reaction faster and for this case the percentage used was 1% bacterial amylase and 3% Fungal was used. In order to find the optimal temperature we had to conduct an experiment on this two amylases. By observing the effect temperature has on the amylases we found the optimal temperature. As the temperature increases the reaction the amylase is undergoing increases therefore the…

Figure 1 shows a set of data that was collected to show the affect of time on the production of pink/gold melanin. The pink/gold melanin is absorbed in a spectrophotometer set at 475nm, which reflects the activity rate of polyphenol-oxidase. Polyphenol-oxidase is found in the potato extract in phosphate buffer ph6, which influences the production of melanin due to the interaction of 0.1% catechol and phosphate buffer pH6. The purpose of the phosphate buffer pH6 is to mimic the cell’s cytoplasm.…

The results of the lab show the decomposition rate of H2O2 at various amounts of KI, H2O, and H2O2. In addition, the effect of temperature on the rate of the reaction can also be seen in the results. Figure 1 Figure 2 `Figure 3 Figures 1,2 and 3 represent the results obtained from the keeping the amount of KI constant and varying the amount of H2O2 and water. It can be seen that when H2O2 is doubled (Figure 1 to Figure 2) the rate is also doubled from 0.0368 mL/s to 0.0723 mL/s. When the…

For part 2 of the experiment (please refer to next page for Figure 3), Figure 3 shows a graph comparing the absorbance readings of the Glutamate-pyruvate aminotransferase serum with the time in minutes. A similar rate was predicted to occur for both the assays done. This would be characterized by the readings showing two parallel lines on the graph. However, the two first readings did not demonstrate this. This could possibly be due to different and insufficient amounts of NADH+ being added to…

Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells. They are vital for life and serve a wide range of important functions in the body, such as aiding in digestion and metabolism. Some enzymes help break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are highly selective…

The main objective of this lab was to observe the effect of the enzyme concentration versus the reaction time of an enzymatic reaction, and the effects of the reactants and products concentration direction of the reaction. Specifically, in this lab the enzymes salivary amylase and phosphorylase were looked at the effect of the enzyme concentration versus the reaction time of an enzymatic reaction were observed by the iodine test along with the benedict’s test. Enzymes are known as biological…

Assignment 1: Discussion Assignment Discussion Question 1 What is the difference between biochemical reactions in healthcare? Biochemical reactions occur as a chemical reaction, which naturally takes place in a living organism. It happens within a cell involving a catabolic reaction (breaking down of molecules) or a anabolic reaction (building up of molecules). The process involves a diversity of biomolecules interacting with one another in order to make up a new biochemical for a particular…

The hypothesis that rate of respiration of chickpeas is highest at room temperature is not supported by the data, although there was error in the data. The data from the lab showed rate of oxygen consumed to be the highest at 1.5 degrees Celsius with a rate of .0124ppt/min/g. At room temperature there was a rate of .0077ppt/min/g, and at the hottest temperature (95.6 degrees Celsius) a rate of -.0131ppt/min/g, meaning that oxygen was not consumed but actually increased in concentration in the…

Enzymes are protein molecules that occur in all living things on earth and enable chemical reactions. Enzymes not only provide an alternate pathway for a chemical reaction, with a lower activation energy, they also bring substrates together in an optimal orientation, thereby speeding up the rate of reaction (Slide Share 2016). In an enzyme catalyzed reaction, the substrate (reactant) binds to the active site and forms and intermediate substance known as the enzyme-substrate complex with the…