Catalysis

In non-competitive allosteric inhibition, inhibitor molecules bind to an enzyme at the allosteric site. Their binding induces a conformational change that reduces the affinity of the enzyme's active site for its substrate. The binding of this allosteric inhibitor changes the conformation of the enzyme and its active site, so the substrate is not able to bind. This prevents the enzyme from lowering the activation energy of the reaction, and the reaction rate is reduced. However, allosteric…

The hypothesis for the experiment is that if the temperature and pH increases (but not past optimal levels), and the higher concentration of amylase, then it will result in faster rates of enzyme activity. Therefore, if the opposite were to occur, then the reaction will slow down and not function properly. For concentration, my hypothesis was proved to be correct. The undiluted solution had higher frequencies of collision at the active site of the enzyme and substrate. Which means the percent of…

The purpose of this experiment was to examine the effects of different types of enzymes on different substrates, and how pH influences the ability of enzymes to breakdown proteins. Starch and albumin were used as the substrate to test the enzymes amylase, pepsin, trypsin, and unknown enzyme T. Lugol’s solution was used to test for the presence of undigested starch, while Biuret solution and Ninhydrin solution were used to test for the presence of undigested protein and amino acids respectively.…

Purpose: To test the effect of inorganic and organic catalyst concentration and surface area on the reaction rate. Introduction: In this experiment the experimenters will seek to determine what factors determine the rate of a reaction with a catalyst. A catalyst is a molecule that starts a chemical reaction, but it is not part of the reaction it is causing. The experimenters will use pieces cut up potato as an organic catalyst. The potato contain enzymes that will perform the reactions.…

Abstract: An enzyme, a protein, is a catalyst, which increases the rate of the chemical reaction. The results of this experiment showed how different temperatures could affect the chemical reaction between the enzyme and the hydrogen peroxide. The temperatures tested in this lab were 30, 35,40,45 Celsius, which are close to the average body temperature which is 37 Celsius. Catalase is an enzyme which breaks down hydrogen peroxide into oxygen and water. Different temperatures were measured to see…

Background: A catalyst is a substance that increases the rate of a chemical reaction without itself undergoing permanent chemical change. The most common catalysts in biological systems are special proteins called enzymes. Enzymes are long chains of amino acids that have taken a very specific three-dimensional shape called a globular shape. This specific shape contains an area that is designed to match a specific molecule. The substance that an enzyme operates on is known as its substrate.…

Enzymes Two major factors influence how effective an enzyme is by promoting the rate of reaction. Each enzyme has a specific temperature that allows it to perform the most work. If an enzyme is placed in an environment under its optimal functioning temperature, the speed of the reaction will slow. Reason being, the molecules of the reactants reduce in energy from the low temperature, less movement means less reactions with the enzymes. At higher temperatures, molecules within an enzyme increase…

Reviewing my data table and graph results it is clear to see that as the substrate concentration increases so does the rate of reaction. The catalysing of enzymes involves molecular movement of the substrate and enzymes in an aqueous solution. Therefore the substrate and enzymes are moving randomly within the solution (Brownian motion). By increasing the concentration of the substrate particles the rate of reaction is increased as the substrate is much more likely to find an active site. This…

The Effects of Competitive Inhibitors on Catechol Oxidase Enzyme Activity. Jaley Willingham, 2015, Functional Biology, Texas State University, San Marcos, 78666. Abstract Competitive inhibitors may have an effect on enzyme activity. We investigated the effects that parahydroxybenzoic acid has on the enzyme activity of catechol oxidase. For this experiment, different levels of parahydroxybenzoic acid concentration were added to catechol oxidase. Each level of concentration was then tested for…

Catalysts are substances that increase the rate of chemical reactions without itself undergoing any permanent chemical change. It speeds up the reaction by lowering the activation energy required. An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. Enzymes carry out the thousands of chemical reactions that occur on cells, or the basic units of structure and function of an organism. Usually, they are polymers of thousands…