Enzyme Lab

Superior Essays
The objective of this lab was to see how enzymes were used, use the scientific method, and how enzymes are affected by temperature.
Introduction
Proteins which constitute enzymes in their final form are macromolecules that make up large amounts of living organism which are called polymers which are made up of many different monomers. An enzyme is a biological catalyst that increases the rate of a reaction by lowering activation energy. Activation energy that the enzyme uses id an energy barrier that must be overcome before a chemical reaction can proceed (Lecture, 4/28). Enzymes are very specific and only work with one particular substance called a substrate molecule using an activation site, this is called the Lock and Key Theory (Lecture
…show more content…
With the first test tube, 0 degrees, add 10 drops of the enzyme solution (the teacher will provide the enzyme). Pour the contents from test tube one into a clean 250mL Nalgene bottle and swirl it. Place the O2 Gas Sensor into the bottle making sure it is tight and nothing is able to leak out. After putting the sensor in the bottle, wait 30 seconds and then start collecting data. Data should be collected for 180 seconds. Once the 180 seconds are complete, remove the sensor from the bottle, rinse and dry the bottle. Make sure that the neck of the bottle is dry so that the O2 Gas Sensor does not get wet. With the sensor, preform a linear regression to calculate the rate of reaction. To do this, choose Curve Fit form the Analyze menu, then select Linear for the Fit equation. Record the value of m as the reaction rate in Table 1. Next, move on to the next test tubes. Use the directions above to complete the test tubes for 27 degrees, 37 degrees, and 55 degrees. Note: when it’s time to do the test tube for 55 degrees, the instructor will give you a special enzyme that has been heating. The results from my lab are as following; test tube 1, 0 degrees, had the lowest rate of oxygen at .029353, test tube 2, 23 degrees, had the highest rate of oxygen at .040048, test tube 3, 37 degrees had an oxygen production rate of .039904, and test tube 4, 55 degrees had an oxygen production rate of …show more content…
It says that once you hit room temperature, the “heat” you are adding to the enzyme is making it denature, or start to lose its shape and function. We know that once it loses its shape and function, that enzyme is “dead”and it can’t do what it is supposed to do anymore which means that our cells are not going to be functioning the way they are supposed to. Remember though, once an enzyme starts the denature process, it cannot be reversed. This is why we see the rate of oxygen production decreasing after we go over room temperature. As we were going through the lab, there were a couple things that we could have done differently. There were a couple problem we faced, but if we were to do it again we would be able to change that. One thing that went wrong was when we were trying to test the regular water bath test tube, 37 degrees, our O2 Gas Sensor wasn’t working correctly, so we had to go back and do it again. If I can remember correctly, when we went to do it again, I don’t think we waited the full ten minutes before we tested it. Also, I believe that on one of the test tubes, we added too many drops of the enzyme which could definitely affect the outcome of oxygen production. If we had to do this lab again, we would be a lot more careful about how many drops of the solutions and enzymes we added. Also,

Related Documents

  • Improved Essays

    After about 5 minutes, turn off the aspirator system until acid fumes can be formed only in the top Exhaust system. Continue the digestion process until the formation of green clear sample in tube. Upon completion of the digestive process, move the rack from tubes to refrigerate the tube vertically for 10 to 20 minutes. After that, add 75 mL of distilled water carefully into the tube which has been cooled followed by the 50 mL of 40% sodium hydroxide, NaOH. In order to prepare the distillate samples, 25 mL of 4% boric acid with 10 drops Bromocresol green indicator are added to recipient solution in a 250 mL conical flask and subsequently placed into the Kjeltec 2100 Distillation unit (Gerhardt, Germany).…

    • 1632 Words
    • 7 Pages
    Improved Essays
  • Improved Essays

    Camphene Synthesis

    • 1013 Words
    • 5 Pages

    A thermocouple was periodically placed on the outside of the flask to make sure that the mixture did not reach past 50 °C. Once the flask was left to mix for fifteen minutes, a strip of starch-iodide paper was inserted into the mixture to determine whether chlorine (Cl2) was present. The mixture was then left to stir for another fifteen minutes. The flask was then chilled in an ice bath to prepare for filtration. A vacuum filtration apparatus was set up using a 150-mL filter flask and a Büchner funnel.…

    • 1013 Words
    • 5 Pages
    Improved Essays
  • Superior Essays

    The alternate hypothesis for this experiment is that if cellular respiration is occurring within the test tube, then the potassium hydroxide level will drop within the pipette because it is absorbing the carbon dioxide. The temperature has the effect to slow down…

    • 1545 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Catalase Lab

    • 1154 Words
    • 5 Pages

    Introduction/Hypothesis Enzymes are catalysts used to speed up reactions and reduce activation energy. These enzymes are important to many bodily functions. Catalase is one of the enzymes essential to cellular health within the body. It is responsible for the breakdown of hydrogen peroxide which, if it builds up, is extremely toxic to cells. One question that scientists have asked and a question that we explore in this lab is what are the optimal conditions for this reaction to occur?…

    • 1154 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    Consequently, five test tubes were prepared at different temperatures to observe which test tube's temperature is the optimal temperature of the enzyme. These are the test tube and their temperature; Tube 1- was blank, Tube 2- room temperature, Tube 3- 35° C, Tube 4- 45° C and Tube 5- 55° C. These tubes had 1 mL of the substrate mixture and 1 mL of pH 6 buffer. These tubes were placed in a spectrophotometer to measure their reaction. This data was collected by placing a timer and acquiring the reaction every five seconds for a minute. (see pg.…

    • 1134 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    After the spectrometer has been blanked begin adding the catechol. Add four-five drops of catechol to each cubit, one at a time, so the other solutions will not contaminate or affected by other properties. The first sample will be placed into the spectrophotometer and record its absorbed wavelength throughout a time period of ten minutes. Repeated this process four times, each time with a change in temperature. Results Depicted in figure 1 are the values that were collected throughout this experiment shown on a graph.…

    • 1101 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Amylase Lab

    • 1641 Words
    • 7 Pages

    Amylase Laboratory Report Introduction: Enzymes are a catalytic protein in a quaternary structure, that helps with the acceleration of molecules called substrates to achieve its desired chemical reaction. In this lab we used a specific enzyme called amylase. According to the lab manual, amylase is an enzyme found in one’s saliva or the pancreas to accelerate metabolism and cause a spontaneous reaction that helps with digestion of a substrate called starch. In order for any polymer such as starch to break down for energy, it must create a hydrolysis reaction which is the breakdown of molecules in a body of water. When that occurs, it can then be used for other processes and chemical reactions.…

    • 1641 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Elisa Research Paper

    • 1444 Words
    • 6 Pages

    Make sure that the centrifuge tubes are properly balanced. If the centrifuge tubes do not have pellets at the bottom centrifuge again. Once back in the biosafety cabinet, remove the supernatant at the top of the centrifuge tubes until only the pellet remains. Discard the supernatant into the organic waste flask. Then in a well add trypan blue.…

    • 1444 Words
    • 6 Pages
    Superior Essays
  • Superior Essays

    When given an unknown solution you can use Excel and determine the concentration or absorbance and determine what the substance is. The results in part two shows that temperature, pH,and concentration can affect enzyme activity. This means the environment has a key role in if the enzyme activity will decrease or increase. Every enzyme assay we did was wrong due to the fact we did not ice our solution long enough. Improvements for this would be to come around to every group and make sure the students are doing…

    • 1525 Words
    • 7 Pages
    Superior Essays
  • Great Essays

    The proteins from each solution will then be precipitated by ethanol and suspended in 2-D cell lysis buffer (). Next, the proteins from the healthy volunteers and the sick volunteers will be stained with CyDye Cy3 and Cy5, respectively, and combined into one solution. The resulting solution will then be subjected to 2-dimensional gel electrophoresis. This will be done by transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting it to a constant electric current (145mA). After the gel has been allowed to run for about an hour, the isoelectric tube will be rinsed with running buffer and set aside.…

    • 1330 Words
    • 6 Pages
    Great Essays