Catalysis

The kinetics of the reaction between peroxomonosulphate with Lactic acid was studied in the presence of Mn(II) at alkaline pH. The reaction followed first order with respect to peroxomonosulphate. The rate of the reaction was studied under pseudo first order condition and found to increases with the [Mn(II)] and lactic acid. Thermodynamic parameters were calculated and reported. Based on the results a rate equation, kinetic scheme and a most probable mechanism has been predicted. Keywords: Alpha…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…

Enzymes are proteins that are a efficient catalyst for a biochemical reaction. It’s responsible to speed up reaction by having an alternative pathway of low active energy. In a simple cell it can be found 2000 different enzymes, each one of these have the ability on catalyzing a different reaction. Each of the enzyme found are responsible on having a reaction occurring in the cell. In this certain reaction in the cell, the cell will tend to make poisonous chemical that could be harmful to the…

Describe the structure and function of a lysosome. Lysosomes are a cell organelle, translating as an independent structure within a cell, possessing a specific structure and function. For a lysosome its functions include; digestion of intracellular or extracellular material, nutrition from digested macromolecules and defence/protection from harmful substances. Lysosomes are variable from cell to cell in terms of shape and size but are conventionally spherical or oval shaped, 0.5µm in diameter…

The hypothesis of the experiment was that the molecular weight of N-acetyl-β-D-hexosaminidase would be around 28,000 kDa but to determine the molecular weight the concept of SDS-PAGE had to be understood. The movement of any charged particle through an electric field is determined by its net charge, its molecular radius and the magnitude of the applied field (7). Being that natively folded proteins are not molecular weight dependent, the proteins would be traveling at different speeds in an…

Catalysts and Inhibitors work by providing an alternative reaction pathway for the breaking and remaking of bonds in chemical reactions, changing the speed of the reaction. They are neither consumed in the reaction. Rather, they alter the amount of activation energy required. Different reactions require different catalysts, as well as different reactions require different inhibitors. The reaction energy path controls the speed of the reaction. The molecules follow the path of least resistance,…

Objective: To understand the function of an enzyme when it undergoes different conditions such as temperature, PH, and concentration of the enzyme. Introduction: In our lab “Enzyme Action” we were testing the enzyme reaction with different substrates. In our lab we tested 3 different enzymes and substrates to find out when they are at the highest potential. Enzymes are necessary for life because they keep our bodies chemical reactions at a normal pace. Enzymes have the ability to keep a human…

Escherichia coli, a bacteria with an optimal pH of between 6.5 to 7.0 to enhance its proliferation is very commonly used in laboratories and studies worldwide due to its low cost and high effectiveness and will therefore be the bacteria of choice for this investigation. It has been shown in many studies such as the article “HdeB chaperone activity is coupled to its intrinsic dynamic properties” very recently published in the prestigious magazine Nature that bacterial growth and overall…

The Effects the Decomposition of Hydrogen Peroxide An investigation into how the amount of oxygen is produced by changing one variable effects the decomposition of Hydrogen Peroxide in the presence of the enzyme. This shows us that when the H2O2 has reacted with the catalyst this is what it produces. There are many ways in which the amount of oxygen produced could be affected. I believe that, surface area of the chip, temperature of liquid that is reacting, the concentration of the liquid…

Zinc is a trace mineral that is necessary for growth, healing, and overall health. It helps more than 100 enzyme systems. It is used in metabolic reactions in cells. Zinc aids in immune system function, the growth of bone, teeth, and connective tissue, and gene regulation. Meat, fish, and poultry contain the best-absorbed form of zinc. However, fortified cereals and legumes also contain zinc. Zinc can also be consumed by taking a supplement. A deficiency in zinc can cause growth…