Spectroscopy

the solution. Scientists accomplish this by using the technique of spectroscopy; spectroscopy can be defined as the science affiliated with the spectra produced when matter interacts with light. Lab 7 investigation 19, “What Factors Affect the Intensity of Color?” expands on the process of spectroscopy, and its relation to the molarity of a substance. The goal of this lab was to determine the effects of the intensity of color of a solution (2). Spectroscopy is frequently used in the medical field by using the process of infrared spectroscopy, which is used to examine biological fluids. This can…

criminal investigations, which can be aided by infrared spectroscopy. IR spectroscopy deals with the infrared region of the electromagnetic spectrum. This region is light with a longer wavelength and lower frequency than visible light. (Wikipedia) IR Spectroscopy is a method to identify substances based on their absorption of infrared wavelengths. It is a study of how the molecules of substances can absorb infrared radiation and convert it into heat. (Cole) A machine called an infrared…

Types of Spectroscopy used in Organic Chemistry 1.1 Introduction Spectroscopy is the study of the interaction between matter and light (electromagnetic radiation) (Crouch & Skoog, 2007). It is often phrased as the light of knowledge (Reusch, 2013). In the past, spectroscopy started by the study of visible light dispersed according to its wavelength by a prism (Anon., n.d.). Now, with the knowledge of light having both wave-like and particle-like characteristics, with given frequency or…

INTRODUCTION The purpose of spectroscopy is to observe the relationship between substances in a solvent, and the associated absorbance and transmittance through a range of concentrations (Lothian & Twyman, 1969). However, one must understand the underlying principles of the electromagnetic spectrum in order to analyze the results of the techniques used in this experiment. When one notices the color of an object, they are observing the wavelengths of visible light – 400 nm to 750 nm…

Photoacoustic spectroscopy (PAS) is a tool to study thermal emission resulting from non-radiative relaxation followed by absorption of radiation. PAS was studied primarily by Alexander Graham Bell, John Tyndall, Wilhelm Rontgen and Lord Rayleigh in 1884. For the history of PAS, was not until 1975 that photoacoustic spectroscopy started to be used as a wide range of different applications. This technique provides the following main advantages over the known types of spectroscopy. The advantages…

Spectroscopy is the study of objects, which is represented on the spectrum of color they radiate. An interaction of electromagnetic radiation with matter. Spectroscopy involves the process of absorption, emission, or scattering of electromagnetic radiation by either ions, atoms, or molecules. The process of absorption involves when light is absorbed by electrons that jump to higher energy levels. On the other hand, emission spectra are established when the electrons from high energy levels give…

Light spectroscopy has become an incredibly useful tool in analyzing the structure and composition of compounds. Specifically, the principles of absorption, the process by which an electron absorbs radiation to excite from a lower energy state to a higher energy state, and fluorescence, the emission of photons when a material is subjected to radiation, allow for the identification and analysis of unknown concentrations of compounds.1 With that being said, the purpose of this experiment is to…

The only compounds that were used for this plate were the spearmint oil, standard for carvone, and the carvone product. The solvent was hexane:ethyl acetate (90:10). This, along with the spotted TLC plate, were added to a 150 ml beaker and covered with a watch glass. The solvent was allowed to move up the plate. The TLC was removed and examined with an iodine jar and a UV light. Each spot had its Rf value recorded. A 10 ml graduated cylinder was filled with hexane. Then, two microliters of the…

proud of me after seeing me work with micropipettes and fancy machines like the NanoDrop 2000 software. So, we were assigned the drug Sulfanilamide. We took 0.25mM of the drug and calibrated/standardized in order to create a calibration curve. We separated the two solvents by using a liquid/liquid extraction. That means we mixed our drug with the two solvents and analyzed how much was in both. We then solved for the partition coefficient (Log P) to describe the degree to which the dug prefers…

or characterized by the use of electromagnetic radiation. Electromagnetic radiation not only includes light (or photons), but thermal energy as well. While the electromagnetic spectrum consists of wavelengths between gamma radiation and the Far IR, most spectrophotometric methods rely on the UV and visible region. In order to use spectrophotometric methods, the sample being analyzed must be able to absorb the energy (photon) used to excite the sample. UV-Vis and other optical instruments measure…