Spectroscopy

Both IR and UV spectroscopy are methods of measuring the wavelengths absorbed by a tested material. IR spectroscopy measures the absorbance of the tested material in the infrared spectrum, the section of the electromagnetic spectrum with a lower frequency than that observed in the visible light spectrum. Conversely, UV spectroscopy measures the absorbance of the tested material in the ultraviolet spectrum, the section of the electromagnetic spectrum with a higher frequency than that observed in…

The ultimate purpose of this lab is to find how much copper is in brass using the visible spectroscopy, in addition to the introduction of Beer’s law, a physical law that can be utilized to determine the unknown concentration of a metal ion in a solution if its absorbance is calculated; you can accurately measure it by means of a standard graph called a calibration curve. Also colorimetry, a technique “used to determine the concentration of colored compounds in solution” (Housecroft, Catherine)…

this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In order to…

This lab module covers the subjects of optical spectroscopy of gas discharges and bond stiffness of materials. Optical spectroscopy can be observed using different types of equipment, such as, a spectrometer, a light collecting fiber, and tubes of certain types of gas (He, H2O, H2 and O2). The data collected displays the peak wavelengths for each of the gases, which can be used to determine the energy released when the electrons change energy levels. Overall, it was interesting to see how…

is to determine the optimal absorption of different solutions, specifically the Chloroplast extract (chlorophyll a and chlorophyll b) and Fast Green solution using the technique of spectroscopy. The following graphs and data will be used to identify the concentration of the given unknown solution #294. Spectroscopy is a process whereby a spectrophotometer is used to determine the absorbance levels of different solutions at different wavelengths (Pettruci et al., 2011). Essentially, it studies…

Abstract In this paper CuO-NH-NH2 nanoparticle was synthesized and used to remove anionic dyes from single and binary systems. The scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDAX) and X-ray diffraction (XRD) were used to characterize the nanoparticle. Direct Red 80 (DR80) and Direct Green 6 (DG6) were used as anionic dyes. The effect of adsorbent dosage, dye concentration and pH on dye removal was evaluated. Kinetic…

Introduction The definition of water hardness, in its simplest form, can be explained as, “the amount of dissolved calcium and magnesium in water.” Water hardness can be attributed to groundwater systems naturally flowing through soil and rock. While doing so, divalent amounts of cations from mineral sources dissolve into the water supply (USGS). Water hardness is not harmful to people’s health. In fact, these minerals can be beneficial, as humans need minerals to survive. However, water…

A spectroscope takes in light and breaks it into their own individual wavelengths, which are the colored lines we see. The light was produced by electrons, which each have their own quantum, or energy level. When we run a current through the atom, the electron gains energy and jumps to a higher level to its excited state. When they fall back to their ground state, they release their stored energy through the form of light. The line spectra for different atoms differ because they each have a…

important for the conclusions that we will make based upon the calculations using the data collected in the lab. Spectroscopy is the study of how matter and electromagnetic radiation interact with one another. In this experiment, we are observing and recording the emission of photons with wavelengths on the visible light spectrum. To understand…

Adhikary, Sujit. “Vermicompost, the story of organic gold: A review.” Scientific Research, Vol. 3, No. 7. Scientific Research Publishing Inc., [circa 2014], cesantaclara.ucanr.edu/files/235723. In the online article, “Vermicompost, the story of organic gold: A review,” the author dives into the benefits of vermicomposting by explaining the basics of the concept, and then digs deeper into the values and assets of this “organic gold.” The article states how vermicomposting not only gets rid of…