Reagent

C were interpreted as positive for denitrification of nitrate to N2. This was observable by the presence of nitrogen gas in their respective Durham tube. Samples containing E. coli and soil B however, required additional testing against reagent A and reagent B as well as the introduction of zinc before determining if denitrification had occurred. Samples containing E.coli, and soil B were interpreted as having denitrification beyond nitrite 2. Cultures or soil samples that produced more gas…

Introduction According to Bio-Rad, Alamar Blue is a “cell viability assay reagent which contains the cell permeable, non-toxic and weakly fluorescent blue indicator dye called resazurin.” (Bio-Rad, n.d.). Meaning that the resazurin is going to be using an oxidation-reduction (REDOX) indicator that will undergo a color change due to the response of the cellular metabolic reduction. “The reduced form resorufin is pink and highly fluorescent, and the intensity of fluorescence produced is…

Amino acid sensitive reagents can be used for detection of latent fingermarks only if the evidence is not wet (as water may wash away the amino acid residues). [5] The usual procedure of using amino acid sensitive reagents on evidence (porous substrates) involves dipping or spraying the items of interest with a solution of the reagent, followed by the application of heat and pressure. [1, 3] The first amino acid sensitive reagent to be used for the detection of latent fingermarks…

Materials: Microscope Bunsen Burner Beaker Test Tube Holder Test Tube Clothes pin Striker Loops and Needles Distilled Water Crystal Violet Alcohol Safranin Iodine Methylene Blue Hydrogen Peroxide Reagent A (sulfanilic acid) Reagent B (dimethyl-alpha-napthylamine) Kovacs Reagent EMb plate Blood plate MSA plate Starch plate Litmus milk tube Nutrient agar Slant…

• Bacteria, are single-cell microorganism with no nucleus that had a sphere, rod, and spiral shape and branching threads. Ranges in diameter between (0.5- 1) µm and in length between (2-4) µm however, they are either a parasite or live independently they are found in all living things and in all environments. Some bacteria in drinking water can cause disease such as salmonella, total coliform (including fecal coliform and E.coli), Shigella, Legionella, and Campylobacter and “their presence…

specific alcohol by using the Grignard reaction. The product is then determined by spectral and physical properties. Introduction The Grignard reaction is one of the most important reactions in organic chemistry. The reaction involves the Grignard reagent, which is formed…

method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5. 2,4-dinitrophenyl hydrazine reagent (DNPH) : 200 mg of…

generally occurs as follows: 1. Add reagent (“titrant”) to a burette [include alcohol, SO2, a base and I2 ] 2. Add sample solvent to the titration vessel 3. Begin stirring the vessel 4. Zero the instrument by titrating unwanted moisture in the system 5. Add the weighed sample to the titration vessel 6. Begin adding reagent from the burette while stirring 7. When the endpoint is reached, the electrode will detect no change in current upon addition of more reagent 8. By knowing how much…

The purpose of the experiment was to observe limiting reactants by using a fixed amount of one reagent along with a varying the amount of the second reagent used in a chemical reaction. Through these results, the concept of limiting reactants can be determined. Using a graph to demonstrate the relation between the volumes of hydrogen gas produced versus the moles of the reagents will help figure out the stoichiometric balance required. The success of the experiment will be determined by the…

After, then other biochemical tests were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria…