Reagent

for results. We could also check the solubility by dissolving a bit of the compound in different acids, bases, and other liquids; KMnO4 is soluble in acids and water but not bases. Then, we moved on to our anion test with different compounds and reagents to see what compounds would create a specific reaction. Whether or not a reaction would happen would indicate the presence of anions; for example, we took a pinch of sodium sulfate and added it to a test tube, added 1mL of hydrochloric acid, and…

enzymatic activity of succinate dehydrogenase (SDH) in various solutions, specifically those containing and not containing a reagent. The assigned reagent was malonate, which was previously hypothesized to be an inhibitor of SDH. Bovine liver was manipulated primarily through centrifugation, and mitochondrial fractions were then created to serve as the enzyme-rich solutions the reagent would act upon. To determine the protein concentration of the mitochondrial fraction, the Bradford assay was…

in different samples through the use of Benedicts reagent and Lugol’s solution. Monosaccharides have a double bonded oxygen atom that forms in one of its two possible chemical groups known as ketone or aldehyde. Disaccharides are two monosaccharides joined together by a glycosidic bond. The Benedicts reagent reacts with the double bonded oxygen atom in monosaccharides by reducing the copper sulfate found in the reagent. Therefore, Benedicts reagent is used in this experiment to detect the…

reducing sugars by testing for a reaction with Benedict’s reagent. The test is positive if the mixture turns yellow or red, but it is negative if it turns blue or green. The Iodine test is performed in order to determine the presence of starch in Unknown #1103 by testing its…

Benedict’s, Lugol’s, Biuret, and Sudan IV tests and are each used to identify an organic compound. For instance, Benedict’s checks for reducing sugars, Lugol’s for starch, Biuret for protein, and Sudan IV for lipids. When testing the unknown with each reagent, the substance only tested positive for reducing sugars. Based on the results of this experiment and what was known about the possible unknowns it could have been, it was concluded that the unknown substance was apple juice. Introduction…

Because the initial trial contained the reagent, and that was set as the baseline, each of the following trials’ reagent was accounted for; therefore, the final absorption numbers subtracted the reagent number and only read the phosphoric acid absorption numbers. Had the calibration trials been performed initially with water instead of the blank reagent, the results would not have been accurate because the reagent itself absorbs some of the light from the spectrometer. Instead…

Introduction and Purpose: Carbohydrates are one of the main types of nutrients, and the main energy source of the human diet. It can be found in a wide range of both healthy and unhealthy foods such as bread, bean, cake, popcorn, cookies, and soft drink (Harvard.edu). The consumption of bad carbohydrate that increases your blood sugar should be limit. If the blood glucose stays high for too long, it can develop type two diabetes (womenheath.gov). In addition, carbohydrates are the most…

The tubes are centrifuged and the fat rising into the calibrated part of the tube is measured as a percentage of the fat content of the milk sample. Apparatus: - Butyro meter, 10.75ml pipette,amyl alcohol, stoppers, gerber centrifuge, water bath. Reagents:-Sulphuric acid, amyl alcohol. Procedure:-  Transfer 10 ml of sulphuric acid in to butyro meter .  Warm the sample approximately to 270C and mix thoroughly but do not shake it so vigorously as to cause churning of the fat. Allow the sample…

A macromolecule is a molecule that contains any "large number" of atoms. There are four main different categories of macromolecules that make up our body: carbohydrates, lipids, proteins, and nucleic acids. (1) Carbohydrates are sugars that store and transport energy. (2) Lipids also store energy and also are signalling molecules. (3) Proteins are linear chains of amino acids that help with the structure, function, and regulation of the body’s tissues and organs. (4) Finally, Nucleotides make up…

In a modular design: An auto analyzer is designed by assembling pulling together of all parts. Closed system In this system the operator has to buy chemicals only from a particular company, because reagents from different companies will simply not be accepted by the machine, so machine will not run. -Modular system in auto analyzer: Compare it with modular kitchen where there are many smaller cabinets fitted according to its use. -Integrated system…