Reagent

Chemical Aspects of Digestion Lab Report By Abdulelah Almutairi Animal Form and Function Lab, 03, 12:30 PM Instructor: Melanie Gustafso-Ropski – Lab Assistant: Corinna Monday March 9,2015 1. Part A • The enzyme used in this experiment is pancreatic lipase. The substrate for pancreatic lipase is lipids or triglycerides. Hydrolysis of triglycerides occurs in the duodenum and the PH rages are from 7.1 to 8.2. • Phenol red was used as a PH indicator of the samples.…

6. Quantitative determination of rat peroxisome proliferators activators γ (PPAR-γ): Rat PPAR-γ concentration was determined by ELISA; using a commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer…

The cyano-ethoxy carbonyl protection Manoharan et al. (1999) developed 2(cyanoethoxycarbonyloxy)succinimide S.27a a stable, crystalline, and convenient reagent for the protection of pendant akylamines in oligonucleotides (Figure X.X.X). The 2-cyanoethyl (ce) group is the most common phosphate protecting group in oligonucleotides; however, ce and corresponding 2-(cyanoethoxycarbonyl) group were not utilized for the nucleobase protection until 2000. Merk et al. (2000) developed…

It is used to detect the presence of reducing sugars. The food sample is dissolved in water, and a small amount of Benedict's reagent is added. During a hot water bath, , the solution should progress in the colors of blue (with no reducing sugar present), orange, yellow, green, red, and then brick red precipitate or brown (with high reducing sugar present). A color change would…

add two-thirds of water then add 4 pipettes of Lugol’s reagent to the beaker. Place the dialysis bag in the large beaker with the open end over the edge then attach a rubber band. Wait about an hour to note any color changes between the beakers.…

top of the bottle to remove any acid and shake well until the precipitate has dissolved. 7. Titrate a volume of treated sample which corresponds to 200 mL of the original sample. This corrects for the loss of some sample during the addition of reagents. This volume calculated using the formula: mL of sample to titrate = 200 x [300/(300-2)] = 201 mL 8. Pour 201 mL of sample from the BOD bottle into an Erlenmeyer…

Benedict 's reagent to each large test tubes and the initial appearance of each solution in the test tubes will be recorded. Then we place each test tubes that add the Benedict 's solution into the hot boiling water and waiting for 5 minutes to get the results. After 5 minutes, take all the test tubes that have Benedict 's out of the hot boiling water and wait for a minute let them be cold. Then shake all the test tubes were cold to shake lightly so that the solution and the Benedict 's reagent…

swimming pool inspection, I learned how to use the reagents for checking the level of (chlorine, PH, alkalinity, sanitizers, and calcium hardness). I learned the standard temperature for water in swimming pool. I learned about the rules of keeping and storage the chemical substances such as chlorine and reagents. I learned how to check on the water tanks and filters. Ms. Laura let me to examine the water statues in swimming pool by using the reagents. In a restaurant inspection, I learned about…

PCR; however, PCR sample A did not show any band. It was concluded that the DNA gel electrophoresis was not successful. Some error occurred during the PCR process, and it could be improper mixing of the reagents, since the amount of each reagent was small. Improper mixing would lead to a missing reagent, and therefore PCR would not turn out right. Another error could be that the concentration of DNA was low, so there was not enough DNA to migrate through the…

diethanolamine, ratio is 1:1, the solution will be transparent; due to the effect of diethanolamine which increases the pH of the solution, make it basic. Dissolving zinc nitrate in 2-propanol is much easier than zinc acetate, therefore adding another reagent like diethanolamine is not necessary. The result is a clear transparent solution without any precipitations. For both the zinc acetate and zinc nitrate solutions, will be stirred using magnetic stirrer for around 20 min for zinc nitrate…