Reagent

assay was to determine if there was any active trypsin by employing Benyzol-DL-arginie-p-nitroanilide (BAPNA), which forms an observable yellow color (from p-nitroaniline) when cleaved by active trypsin. The second assay used biorad protein assay reagent to estimate the concentration of the protein that was eluted. Both assays required a spectrometer to observe the color changes by absorbance. The hypothesis is that active and inactive tryspin would be able to be separated by affinity…

chiral auxiliary is introduced and then removed once its proposed is fulfilled. In the case of the third generation or reagent controlled methods, the two extra steps needed in the second generation methods to attach and remove the auxiliary can be avoided completely by using this method. This method converts an achiral substrate to the chiral product by the use of a chiral reagent. S ---R*--- P* The control is now intermolecular whereby the first and second generations were intramolecular.…

I was only off by one point for the boiling temperature. As far as the classification test my accuracy remained constant, my results from the Lucas test indicated I had a primary alcohol given there was no change occurring when I added the Lucas reagent. For my IR spectrum, I have confirmed C-H stretches at absorption levels of 1,370, 970, and 985. An O-H absorption occurred around the 3,000-3040 vibration making indications that there was an alcohol present. My NMR gave triplet and singlet…

was set to boil on a hot plate while samples of serum and benedicts reagent were collected. Six test tubes were gathered and labeled by which blood vessel the serum was collected from and by which category, either postprandial or fasting. Then by using a metric ruler two measurements were placed on the bottom of each tube, one at one centimeter and one at two centimeters. This was to indicate how much serum and Benedict’s reagent to use, serum to the first mark and Benedict’s to the second…

saturated thiocyanate was mixed and diluted to 100 mL in a volumetric flask with 0.5 M HCl. The absorbance was read over a period of 2 hours at 30 min intervals. Excess Reagent Standard iron solution was used and 0.5 M potassium thiocyanate was prepared. Following the reagent table below, the corresponding amounts of each reagent was added to 100 mL volumetric flask and diluted with water. The samples were analyzed with water as the…

product being fully saturated. Metal hydride reducing agents that have different reactivities toward specific functional groups are commonly used in chemical reductions. There are challenges in synthetic organic chemistry to develop new reducing reagents that react with only one type of functional group in the presence of other reducible groups. The two most common metal hydrides…

A. Molar Mass C. Particles B. Stoichiometry D. Mole 3. The first step in every Stoichiometry Problems is to? A. Balance the equation C. Solve the mass of the element/compound B. Determine the Limiting Reagent D. Determine the Excess Reagent 4. Stomach acid is made up of hydrochloric acid (HCl). How many percent by mass does hydrogen take up in the compound? A. 27% C. 1.6% B. 2.8% D. 2.0% 5. A piece of marble contains 8.74 x 1025 formula units…

Latent fingermark being imperceptible to the unaided eye is the decisive form of physical evidence found on divergent surfaces at the scene of crime. It is decisive because of its perpetuality and uniqueness. The word ‘fingerprint’ is common in commonality but individual to a person. When a person grasp something, he left the traces of residues (secretions of glands) conforming impression of the ridges of the fingers. The fingermark residue is a complex matrix of secretions and xeno-materials.…

Chemicals: Folin-Ciocalteu reagent, sodium carbonate, gallic acid, trifluoroacetic and sulphuric acids, Trolox (6-hydroxy -2, 5, 7, 8- tetramethyl chromane-2-carboxylic acid), ABTS (2, 2'-azinobis (2-methylpropionamidine) dihydrochloride), 2′, 7′-Dichlorodihydrofluorescein diacetate (H2DCFDA), dexamethasone (DEX), and lipopolysaccharide (LPS) were purchased from Sigma Inc. (St. Louis, MO). Catechin, epicatechin, procyanidin B1 and B2 were purchased from Chromadex (Irvine, CA). Dulbecco’s…

The scientific context of the Antimicrobial Susceptibility test is to gauge the viability of antimicrobials and other chemotherapeutic agents on pathogenic organisms in recommending a suitable treatment. The scientific context of the the API 20E test is clinically used in quickly identifying enterobacteriaceae bacteria and gram-negative rods. In the Antimicrobial Susceptibility experiment, three microorganisms which were Staphylococcus aureus, Escherichia coli, and the unknown were put into…