Reagent

had dissolved completely. It was emphasized that excess ammonia should be avoided, since it could affect the results of the test by affecting the production of the Tollens’ reagent, and it was also emphasized that these solutions should be added in this exact order. After adding the drops of 2% aqueous ammonia, the Tollens’ reagent was completed, and 2 drops of every respective compound were added and the test tube was shaken. The test tubes were allowed to sit for 10 mins, and in those 10 mins…

into each well. The plates were incubated for 48 hours for the cells to stabilize and interact with compound. After that, MTT assay was used to determine the viability of the cells. 10 µL of MTT reagent was inserted into each well. The plate was incubated for 2-4 hours more. Then, 100 µL of detergent reagent was added. The plates again were incubated for 2-4 hours. Finally, the plate was read at 570 nm using a spectrophotometer. The graphs were plotted of the percentage of cell death versus…

subtracting the secondary absorbance reading at 700 nm. For the Cobas c501 module, this results in an increased reagent background absorbance to a level which exceeds the maximum absorbance specified for the instrument detector (32000 ABS), once reagent 3 (NADPH) is added to the reaction (Figure 1). In 2014, Roche released a technical bulletin [8], advising customers to allow the reagent rest open on the shelf for 24 h to release ammonia eventually built up, prior to loading on the instruments.…

μL. The test tubes were then left to soak in a 37°C water bath before having 2 mL of 1 M hydrochloric acid added to a test tube of pure amylase solution. Lugol’s iodine was used to test for the existence of starch in the samples, while Benedict’s reagent detected any broken-down glucose. The same procedures were then performed waiting 5, 10, and 15 minutes before deactivating the amylase. Then again repeating the procedures with 4°C and 95°C water baths. Five test tubes were filled with pH…

To ensure the reaction was complete the melting point was obtained and determined to be 128℃. The limiting reagent for this reaction was determined to be benzaldehyde as it was a 2: 1 ratio. Using the theoretical yield of 20.70 g, the percent yield was calculated to be 28.74 %. This could have been higher if the reflux was completed for 90 minutes like the procedure…

D-glucose stock) were taken in a series of test tubes and the volume was made up to 1.0 ml. A reagent blank was prepared by taking 1.0 ml water alone. Anthrone reagent (0.5g anthrone and 10g thiourea, in 1L 66% H2SO4) was added to all test tubes to a final volume of 5.0 ml and heated in a 90°C water bath for 15 min. The blue color developed was read at 620 nm in a UV-Vis spectrophotometer against the reagent blank. The amounts of soluble carbohydrates in samples were calculated from the glucose…

relative SDH activity. So if other components of the cell are found with high concentration of SDH it is evident that it was polluted with mitochondria which shows the type of error you make. In our SDH Assay, we manipulated SDH activity by using reagents such as: potassium phosphate (maintains optimum range for SDH and is a buffer to control pH or reaction), azide (blocks electron transport chain to block the build up of FADH2), buffered sucrose (keeps mitochondria from bursting),…

alkaline levels. Next, we will place one of each test tube in the three different temperature water baths for 30 minutes. After 30 minutes, we will remove the test tubes from the water baths and add six drops of Biuret reagent to each tube and mix thoroughly. The Biuret reagent will test for the amount of peptide bonds present in the egg whites. Then we will take a picture of the results and record the color of each tube. A dark purple color indicates a positive test for proteins and a light…

Introduction:The objective throughout the semipermeable membrane experiment was to test the permeability of the caterpillar's intestine. Through this experiment one would be able to observe a transverse of small molecules through the cell membrane. Question: Were starch and Lugol’s molecules able to cross the membrane? Hypothesis: If there is a presence of glucose among the fluids in between the intestine and circulatory system then there is to be a breakdown of starch molecules within the…

two reagent trays for…