Title: Construction of FATTY ACID DESATURASE 2 (FAD2) gene knockouts using RNAi novel technique in the model plant Thlaspi arvense. Abstract: FATTY ACID DESATURASE 2 and FATTYU ACID ELONGASE 1 (FAE1) are the two genes of interest present in the plant pennycress. FAD2 is in the pathway that synthesizes the polyunsaturated linoleic (18:2) and linoleic (18:3) fatty acids from the monounsaturated oleic acid (18:1). Polyunsaturated fatty acids are undesirable in biodiesel because they confer oxidative instability, but greater energy (J.C. Sedbrook, et al, 2014). Targeting gene function reduction of FAD2 in Thlaspi arvense using RNA interference method, lowered linoleic/linoleic acid content and increased oleic acid content in seed oil. We are hoping…
The Rf values were obtained by reacting the lipids on the TLC plates with iodine, to visually show how far the lipids moved up the TLC plate. For the identification of the amount of lipids in milk, the milk was combined with an organic mixture of CHCl3/MeOH, to isolate the lipid layer. By centrifuging the organic solvent with milk the heavy lipids sink to the bottom while the water layer which contain water soluble carbohydrates, peptides and small amino acids float to the top. Since the organic…
Soap may be made up from either saturated fatty acids and/or unsaturated fatty acids. The most common saturated fatty acids used in soap are, “lauric acid, myristic acid, palmitic acid and stearic acid” ( Michelle 2014). If a more effective clean is desired then, using a saturated fatty acid is the way to go because they get rid of oils better. Due to this, soaps made up of saturated fatty acids are less prefered for skin because it will take away the natural oils produced by your skin. These…
Mustard oil also is rich in fiber which aids in digestion and helps to relieve digestive problems as well. • Stimulates hair growth: Mustard oil contains high levels of beta carotene and Vitamin A, iron, fatty acids, calcium and magnesium that stimulate the growth of hair and keep it healthy. • Improves heart health: Mustard oil is rich in omega-3 and omega-6 fatty acids which lower the cholesterol levels and promote the good cholesterol which in turn ensure that the cardiovascular health is…
In the foregoing paper, the author reported that ethyl ester of highly unsaturated fatty acid freshly obtained from cuttle fish oil showed quite the same good nutritive effect as oleic acid ester for rat, while the acid exposed to air was proved to be extremely toxic. Since it seems to be most plausible that the toxicity is concerned with the formation of peroxide by autoxidation of unsaturated fatty acid, the author determined the amount of peroxide. The result proved. this was just the case,…
certain fatty acids strip away the nerve cells’ protective layer, causing major brain damage. It is an orphan disease, meaning that there are only a few people that have it, and there is little to no profit incentive for drug companies to develop a cure. In Lorenzo’s Oil, Lorenzo Odone is diagnosed with this disease, which at the time, was nearly always fatal. During Lorenzo’s father’s journey to find a cure for his son’s disease, he discovered that if the intake very long chain saturated fatty…
stimulant (British Pharmacopoeia 2009). • Methyl paraben was generously donated by Arab Pharmaceutical Manufacturing Company. Methyl paraben is methyl-4-hydroxybenzoate, prepared by the esterification of p-hydroxybenzoic acid with methanol. It occurs as colorless crystals or a white crystalline powder. It is odorless or almost odorless and has a slight burning taste (Rowe et al., 2009). Methyl paraben is very slightly soluble in water, freely soluble in alcohol and in methanol (British…
General Introduction Dietary lipids: Dietary lipids consist of a broad group of compounds that are soluble in organic solvents and insoluble in water. Their density is lower than water and consistency varies from liquids to solids at normal room temperature, depending on their structure and function. They are also referred as ‘Oils’ which are liquid and ‘fats’ which are solid at room temperature. Lipids are the important constituents of diet due to their higher energy value. One gram of lipid…
oxygen3. Serratia Marcescens is a gram negative bacterium. Gram negative bacteria have a thin cell of a single layer of peptidoglycan that is enclosed by an outer membrane. The outer membrane is surrounded by special phospholipids composed of fatty acids called lipopolysaccharides that are attached to a glucosamine…
neutralization reaction. A neutralization reaction is a chemical reaction in which an acid and a base react to form an ionic compound (a salt) and water; the resulting pH is closer to 7. The general chemical equation for a neutralization reaction is: Acid + Base Water + Ionic Compound This can also be considered as a double displacement reaction. However, since the reacts are an acid (carbonic acid) and a base (sodium hydroxide) and the products are a salt (sodium carbonate) and water (H2O),…