Absorption spectroscopy

Spectroscopy is the study of objects, which is represented on the spectrum of color they radiate. An interaction of electromagnetic radiation with matter. Spectroscopy involves the process of absorption, emission, or scattering of electromagnetic radiation by either ions, atoms, or molecules. The process of absorption involves when light is absorbed by electrons that jump to higher energy levels. On the other hand, emission spectra are established when the electrons from high energy levels give…

Photoacoustic spectroscopy (PAS) is a tool to study thermal emission resulting from non-radiative relaxation followed by absorption of radiation. PAS was studied primarily by Alexander Graham Bell, John Tyndall, Wilhelm Rontgen and Lord Rayleigh in 1884. For the history of PAS, was not until 1975 that photoacoustic spectroscopy started to be used as a wide range of different applications. This technique provides the following main advantages over the known types of spectroscopy. The advantages…

Types of Spectroscopy used in Organic Chemistry 1.1 Introduction Spectroscopy is the study of the interaction between matter and light (electromagnetic radiation) (Crouch & Skoog, 2007). It is often phrased as the light of knowledge (Reusch, 2013). In the past, spectroscopy started by the study of visible light dispersed according to its wavelength by a prism (Anon., n.d.). Now, with the knowledge of light having both wave-like and particle-like characteristics, with given frequency or…

this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In order to…

The purpose of this experiment is to determine the optimal absorption of different solutions, specifically the Chloroplast extract (chlorophyll a and chlorophyll b) and Fast Green solution using the technique of spectroscopy. The following graphs and data will be used to identify the concentration of the given unknown solution #294. Spectroscopy is a process whereby a spectrophotometer is used to determine the absorbance levels of different solutions at different wavelengths (Pettruci et al.,…

Both IR and UV spectroscopy are methods of measuring the wavelengths absorbed by a tested material. IR spectroscopy measures the absorbance of the tested material in the infrared spectrum, the section of the electromagnetic spectrum with a lower frequency than that observed in the visible light spectrum. Conversely, UV spectroscopy measures the absorbance of the tested material in the ultraviolet spectrum, the section of the electromagnetic spectrum with a higher frequency than that observed in…

The question is, “Which paper towel brand is the most absorbent?” The hypothesis is, “If a dropper drops water equally on a paper towel of each brand until it started to drip, then Bounty brand would contain the most water, in grams. The independent variable is the brands of paper towels. The dependent variable is the grams of water that was contained in the paper towel. The control variable was that every paper towel sheet was cut to be 15.2 centimeters by 28.1 centimeters. The control group…

2009). The purpose of this experiment was to observe what the absorbance spectrum of Spinacia oleracea extract was and what the absorbance spectrum of the pigments were within the extract. From the use of the spectrophotometer I was able record the absorption spectrum of chlorophyll a, chlorophyll b, carotene, and xanthophyll to determine the range of wavelengths of light absorbed by the molecules. It was predicted within the Spinacia oleracea extract, chlorophyll a and…

Atomic Absorption is a technique that determines the metals that are dissolved in a given solution by looking at light absorbance. The amount of absorbance in a sample is proportional to the concentration of the metal atoms in a sample. While testing is occurring…

A spectroscope takes in light and breaks it into their own individual wavelengths, which are the colored lines we see. The light was produced by electrons, which each have their own quantum, or energy level. When we run a current through the atom, the electron gains energy and jumps to a higher level to its excited state. When they fall back to their ground state, they release their stored energy through the form of light. The line spectra for different atoms differ because they each have a…