Absorption spectroscopy

Although Fraunhofer discovered the absorption spectrum, it was left to Gustav Kirchoff to explain that dark lines are the result of photons being absorbed as light passes and the reasoning behind the different frequencies in 1859. Now with Fraunhofer lines, astronomers are able to learn a lot…

Each spot had its Rf value recorded. A 10 ml graduated cylinder was filled with hexane. Then, two microliters of the carvone-hexane solution were added to the hexane using a micro pipette. This solution was moved into the crystal container for UV spectroscopy. The UV spectra was taken from 200 to 400 nm and the concentration of the sample was 2.0E-4. The absorbance was recorded at 232 nm and 273 nm. Results The carvone product was weighed to be 1.88 g and appeared to be a mostly clear liquid.…

Adhikary, Sujit. “Vermicompost, the story of organic gold: A review.” Scientific Research, Vol. 3, No. 7. Scientific Research Publishing Inc., [circa 2014], cesantaclara.ucanr.edu/files/235723. In the online article, “Vermicompost, the story of organic gold: A review,” the author dives into the benefits of vermicomposting by explaining the basics of the concept, and then digs deeper into the values and assets of this “organic gold.” The article states how vermicomposting not only gets rid of…

Spectrophotometry is a method in which organic and inorganic substances are identified or characterized by the use of electromagnetic radiation. Electromagnetic radiation not only includes light (or photons), but thermal energy as well. While the electromagnetic spectrum consists of wavelengths between gamma radiation and the Far IR, most spectrophotometric methods rely on the UV and visible region. In order to use spectrophotometric methods, the sample being analyzed must be able to absorb the…

Figure 1 shows the relationship between concentration and absorbance known as the Beer-Lambert law or calibration curve. The data from the made solutions in table 2 was used to create the graph. The graph has a linear trend line that predicts where a point would fall on the graph. The equation of the trend line is equation 7: y=0.9508x-0.0055 with a R2 value of 0.99943. This equation was used to find the true concentration of the made solutions as well as the unknown solution. The R2 value…

Hey Mom, I know you’re dying to know how my semester is going so I thought I’d share with you some of the assignments I’ve been working on. I just finished my first chemistry lab project involving drugs! Yes, drugs, but not the ones you’re thinking about. We worked with Sulfanilamide, Phenacetin, Acetaminophen, and your favorite, Caffeine. The goal for the experiment was to determine where the drugs may reside according to their properties. We learned that there are parts in the cell that are…

1 WRITTEN ESSAY Paragraph 1 The FePO4 crystal is examined at various temperatures ranging between 294K and 1073K by neutron powder diffraction. At relatively low temperatures, FePO4 adopts an α-quartz structure, which is tetrahedral. High pressures causes a phase change to a more dense octahedral structure, which is known as β-phase. The transition temperature is 980K. Cutoffs can be observed during the firstorder transition,. For α-phase, the increases in cell parameters…

week was to figure out the relationship between the wavelength of light absorbed and the color of a compound. Found on page 87 of lab manual, the absorption wavelength is related to the energy of the light that is absorbed by a molecule. The color of the light reveals information about the kind of molecule that does the absorbing. The amount of absorption is dependent on the type of molecule.3 Each of the cuvettes filled with the prepared solution was measured in wavelengths that ranged from 350…

the desired volume of each made solution to create the diluted solution, equation 5: M_C V_C=M_D V_D was used where M is molarity, V is volume, c is concentrated solution and d is diluted solution. Finally, the percent error of concentration and absorption was calculated using equation…

be of great benefit to develop a more effective system to heighten the potential advantages of early diagnosis. Current research involves the use of analytical techniques as diagnostic tools for a variety of chronic illnesses. IR, Raman, and NMR spectroscopy, as well as Mass Spectrometry have been successfully utilized in the detection of brain, bone/joint, and endocrine disorders, as well as genetic disease, syndromes of internal organs, and cancer. These technologies have proven to be more…