Interpretation and Evaluation: Rinsing Process: When performing a volumetric analysis all equipment to be used must be washed with deionised water. This is done in order to remove anything that contaminate your solution and have an effect on the results. It is important that deionised water is used and not regular tap water, this is because tap water could itself contains many unwanted impurities such salts, other solids and bacteria. If these were to contaminate the solutions to be used it…
Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…
were used to help understand the different behaviors of different concentrations. Household items were used to help apply basic theories about acids and bases such as pH. The titration curves were evaluated to help determine which indicator needed to be used for the indicator titration. Anions are ions that have more electrons than protons, therefore,…
The four methods used for quantification of alkalinity (4) are summarized below: •Use of a pH indicator: a change in the color from pure yellow to orange is observed and recorded when a known pH is reached. In this his experiment we used methyl orange as indicator The consumed volume until specific pH allow us to know how much volume is required to reach the endpoint. •Visual identification from titration curve; the data are recorded…
Water Quality Water is essential to human life and the health of the environment. Good water quality is essential for irrigation, drinking and recreation and also sustains ecological processes that support native fish populations, vegetation and bird life. We expect that our water supply will not only be safe, but will taste good, and look crystal clear. Water quality is commonly defined by its physical, chemical, biological and aesthetic (appearance and smell) characteristics. Water quality…
Natural Indicators Lab; a Study in pH Levels Using Green Cabbage Introduction- pH is the concentration of hydrogen in a substance. It is the scale used to tell how acidic or basic a solution is. The normal pH scale goes zero to fourteen. Zero to about six indicates that the solution is an acid. If the solution is closer to zero it makes it a stronger acid, but if the solution is closer to seven than it is a weak acid. If a solution has a pH of seven then it is neutral. A pH of eight to fourteen…
ACID-BASE PROPERTIES OF SODIUM CHLORIDE, SODIUM FORMATE AND AMMONIUM CHLORIDE INTRODUCTION The objective of this experiment is to examine the definitive characteristics of acids and bases by investigating the characteristics of strong versus weak acids; more specifically, why these differences are important in the process of titrations. In this experiment, there are two titrations performed and measured the pH of one salt solution. This experiment includes a strong acid and strong base…
number of mL or moles needed to reach the endpoint, the greater the buffering capacity. This is because it signifies that the highest concentration buffer resist the most change in pH. If a student were to use the bromothymol blue indicator instead of the thymol blue indicator in Part B, he/she would not be able to test the buffering capacity of acetic acid/sodium acetate buffer by adding HCl because bromothymol blue changes color when the buffering…
Conclusion: I predicted that liquid A was glycerine due to a calculated density of 1.38 g/mL. However I was wrong the actual density of liquid A was 1.38 g/mL and the substance was actually corn syrup. Therefore the percent of error for the density of liquid is 1%. For liquid B I had predicted that its substance would be Glycerine due to the calculated density of 1.19 g/mL. I was incorrect for the density of liquid B which was actually 1.26 g/mL, but because the calculated and actual densities…
The two main purposes of the lab were to determine the percent by mass of acetic acid in vinegar and to determine the molar concentration of a strong acid. In both parts of the experiment a volumetric analysis was used. This is primarily the aid of volumetric glassware in order to determine specific measurements and calculations. Potassium hydrogen phthalate was used as the primary acid standard to determine the molar concentration of the sodium hydroxide solution in part one of the experiment.…