Stain

Once dry the microorganisms can be seen under a microscope. This procedure is very different from that of a differential stain. A common differential stain and the one used in this lab is the gram stain which is more complicated the simple stain and uses more than one dye. The procedure involves a primary stain, a mordant that locks in the stain, a decolorizer, and a counter stain. The use of multiple dyes allows the viewer to identify gram negative and gram positive bacteria based on whether it’s pink or purple, respectively. This occurs because of the chemical differences in these two types of bacteria; gram negative bacteria has a thin layer of peptidoglycan in the cellular membrane and an outer membrane and the gram positive bacteria has a thick layer of peptidoglycan in the cellular membrane and lacks an outer…

Fight silverfish in the bedroom Silverfish find ideal living conditions in human habitations. In addition to the bathroom , the bedroom also offers optimal living conditions for the small animals. The insective insects belonging to the insects feel particularly well because of the prevailing temperatures and humidity. Although they are not really harmful to health and in the widest sense even useful, nobody wants to share their home or sleeping with the voracious animals. Often silverfish are…

Introduction The Gram stain is a valuable tool to microbiologists in identifying the cell well composition of prokaryotic species. The underlying process revolves around a series of chemical dyes, and their propensity to bind to peptidoglycan polymers in the cell wall. The results allow scientists to infer properties about the outer coating of an organism and is often the first step in identifying an unknown species. For the purpose of our experiment, the results of the Gram stain will be as…

STAIN GLASS PROJECT CRITERION A: KNOWING AND UNDERSTANDING: INQUIRY AND ANALYSIS. CONCEPTS What is a concept? A concept is simply an abstract or general idea or notion that is derived or inferred from specific instances or occurrences. KEY CONCEPT: CREATIVITY -FACTUAL Factual— Stain glass is a technique that has been present in many cultures for centuries. What is creativity? Creativity is defined as the tendency to generate or recognize ideas, alternatives, or possibilities that may be…

When finding out unknown bacteria you have to go through several steps to figure out what it could be. I was given unknown bacteria number 28 and asked to run different testing to identify exactly what this bacterium is. I ran 6 test to determine my unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have. A gram stain is one of the most…

Internal conflict involves the struggle between an individual and their conscience. People will struggle with their choices, their morals, their faith, and their sense of self. This has to do with individuals having to make wrong or right or even with overcoming emotions or mixed feeling. In our lives, courage is important because we all have to deal with decisions and things we don’t like. In the story “Stains”, “Signs”, and “Button, Button”, the characters face their own internal conflicts…

The permeability of tooth enamel makes it extremely vulnerable to stains. Colored beverages can be absorbed by the teeth, which may lead to a brown or yellow tinge on the teeth. Acidic beverages are another problem for tooth enamel because the acid can actually wear the enamel away and temporarily softens the enamel; thus, making teeth even more prone to staining. Bright Color Beverages Can Stain the Teeth The color in beverages comes from intensely pigmented molecules that are referred to as…

I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

¬¬ ‘Slipping the Punch’ – Roth’s Playful Pugilism in The Human Stain Phillip Roth’s writing is characterised by conflict between a desire to champion the individual over societal codes and conventions, and an almost fatalistic sense that these countervailing forces will always overwhelm individual self-determination and self-expression. This conflict often manifests in, at the very least, an ambivalence about language itself: as Kasia Boddy expresses it in Boxing, A Cultural History, ‘For…

The Editorial, “The Stain of Racism in New York Prisons” by The Editorial Board is about racial bias against minority inmates and also a Parole Board that is not organized. The rhetoric, connotation and bias found in this editorial make it a lot of stronger. Although the bias makes the argument slightly weaker, the use of connotation and rhetoric really strengthens the argument. The article “The Stain of Racism in New York Prisons” contains a lot of rhetoric. This article contains mostly logos…