RNA polymerase

All the animal experiments were performed in accordance with the guidelines established by the Animal Ethics Committee of Wonkwang University, and this experiment was approved by the committee (Approval No. WKU15-101). To evaluate the effect of BGE on fasting blood glucose levels, C57BL/6J (dbdb) 30 mice (20 –25g, 4 weeks, male Lepr(+/+)) and C57BL/6J (dbh) 10 mice (20–25g, 4 weeks, male Lepr(+/-)) were used as the animal model. All the animals were purchased from SAMTACO (Osan, Korea) All the…

In PCR, DNA is combined with several chemicals necessary for the DNA to replicate properly. Special segments of RNA (DNA with only one strand) specific to the gene we want to copy are added to the DNA to act as signals for the enzymes to know where to copy the DNA. Nucleotides and a version of the main DNA-copying enzyme that can work at high temperatures are also…

In addition to PCR, gel electrophoresis is a technique that allows the visualization of separated DNA, RNA, and proteins according to size [5]. Both techniques are advantageous to characterize human genetics by determine differences among individuals. Previous published data of the D1S80 locus from various populations such as in Turkey have observed the…

This study compared the changes in gene expression within wing tissue of Little Brown Myostis infected with White Nose Syndrome to bats without White Nose Syndrome. As expected, Pseudogymnoascus destructans caused significant changes in gene expression within the tissue. Inflammatory responses paired with anti-fungal host responses were triggered by the introduction of Pseudogymnoascus destructans. Necessary chemokines (Small secreted proteins that induce a certain response) were produced in…

to the brain or replicate within muscle” (www.medicalnewstoday.com). “The rabies virus is a rod-or bullet-shaped single-stranded negative sense, un-segmented enveloped RNA virus” (www.ncbi.nlm.nih.gov). “The virus genome encodes five proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and polymerase (L)” (www.cdc.gov/rabies/transmission/virus.html). “The rabies virus occurs in more than 150 countries and territories and is present on every continent with the…

into Agrobacterium tumefaciens using the strains EHA105, GV3101, and EHA105.The transgenic plants were then screened via hygromycin B selection. They were then further verified by the polymerase chain reaction analysis of genomic DNA to detect the appearance of the genes AaIT or GNA. Through this method, messenger RNA expression levels of AaIT and GNA were able to be analyzed. These plants were seen to be morphologically different from the wildtype plants. The transformations done on tobacco and…

Influenza is a highly contagious respiratory infection caused by the influenza virus. The illness can range from mild to severe, and can infect people of all ages, races, genders, and sizes. Generally, the illness is not life threatening in most people, but certain groups are at higher risk of requiring hospitalization or even dying from influenza. These at-risk groups of people include young children, the elderly, and people with compromised immune systems. The CDC estimated that there were “25…

the near future, we expect to see new technologies that will flood law enforcement. Some of these new technologies are the analysis of tissue-specific mRNAs and microRNAs (miRNAs) to identify body fluids deposited at a crime scene, determining which RNAs are expressed in a biological sample can help identify the tissue and potentially help reconstruct or classify a crime; for example, the presence of semen may support an allegation of rape. Law enforcement expects forensic technicians to have…

p53 interactomes measured by ChIPPet. NRSF binding near promoters was concluded to be a repressor of transcription of Jurkat cells. This was found by seeking out high-confidence promoter predictions in 1KB of a ChIPSeq peak and hybridizing labeled RNA to Illumina RefSeq8 Sentrix arrays. A MEME search concluded only 2 candidate motifs when their experimental interactome peak domains were applied to the search. The two motifs from the MEME search were concluded to correspond to the left and right…

Bacteria often contain plasmids, plasmids are circular, double stranded DNA that is not part of the nuclear DNA. Plasmids usually contain genes that code for several traits. Genetic transformation relies on inserting a gene that codes for a new trait in to the plasmid. For this lab, genetic transformation is the insertion of a new DNA in to E.coli cells, the pGLO plasmid was genetically engineered to carry the green fluorescent protein and a gene that codes for a protein that gives the bacteria…