Regulatory Structure Of The Arabinose Operon

Bacteria often contain plasmids, plasmids are circular, double stranded DNA that is not part of the nuclear DNA. Plasmids usually contain genes that code for several traits. Genetic transformation relies on inserting a gene that codes for a new trait in to the plasmid. For this lab, genetic transformation is the insertion of a new DNA in to E.coli cells, the pGLO plasmid was genetically engineered to carry the green fluorescent protein and a gene that codes for a protein that gives the bacteria a resistance to the antibiotic. The genetically modified plasmid can be used to transform the bacteria and so give it a new trait. (Biotechnology Explorer, n.d.). Genetic transformation is composed of a single cell, because in order to transform a single-celled organism, the individual has to …show more content…
Enzymes that are necessarily for the metabolism of the Arabinose are coded by the Arabinose operon. There is a complex regulatory system for the Arabinose operon. The Arabinose operon can be regulates positively and negatively similar to the Lactose operon. Therefore, making the Arabinose operon an inducible operon. When E.coli cells are growing with Arabinose present, there are three enzymes that are part of its metabolism, they are present in the cell in small quantities and they contain no expression of the operon. This is due to the enzymes adaptive mechanisms that make sure that enzymes that are required to catabolize the Arabinose are produced in particular amounts when the Arabinose is present in the environment. Catabolite repression is exhibited by the Arabinose operon. Catabolite repression is a positive control of transcription since regulatory proteins affects the increase in the transcription rate of operons. Arabinose operons will express its genes only if the best carbon source present in the environment is