Methods: Procedures outlined by Dr. John Choinski and Dr. Steven Karafit in Principles of Biology 1: lab notes manual, were followed (Choinski & Karafit, 2016). Temperatures tested included 25, 32, 39, 46 and 53 °C. Each sample was held for 2 minutes at each temperature.
Results: Incubation of yeast catalase provided investigation of enzyme activity. Visual bubbling occurred in each sample with fewer bubbles at 46 and 53° C. A 54% decrease in enzyme activity was seen between control and highest temperature of 53° C. The highest enzyme activity was seen at 32°C, which …show more content…
A 54% decrease in activity was recorded between control and highest 53°C temperature tested (mean±SD). Results indicate enzyme disruption occurs between 46-53°C.