With two tubes labeled -pGLO and +pGLO to indicate which tube was the test and which was the control. To begin pipette 250 microliters of transformation fluid into each tube and then add e.coli plates. Once done close the lids of the tubes and place them on ice. Using a sterile loop remove a single colony of bacteria from a starter plate. In the +pGLO tube submerge the single colony into the transformation fluid, then return the tube to the ice bath. Gain another single colony and insert it into the tube -pGLO and return to the ice bath. Once done, with a new sterile loop collect pGLO and add the loop into the +pGLO tube and mix the pGLO into the transformation fluid. Incubate for 15 minutes. During incubation label four luria bertani broth
