Cellular Respiration Lab

Determining Enzymes that Result to Mutation on the three Stages of Cellular Respiration

Mary A Enriquez
University of the Pacific
November 11, 2014
2 Green
Dr. Geoffrey Lin-Cereghino

Abstract: The purpose of this lab is to determine which type of food source could possibly help strains grow on both permissive and restrictive temperature. Based on all the chemicals accumulating, we can determine the enzymes that are used to inhibit the three stages of cellular respiration.
Introduction: For cells to successfully accomplish its task such as moving and reproducing, it needs energy. Most energy comes from the sun and the energy is stored in organic molecules. Cells release its stored energy by converting chemical energy in glucose
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According to cod.edu, Alterations in key portions of a protein may lead to changes in the specificities of the protein and this is a temperature sensitive mutation. Proteins are not for heat exposure. A temperature sensitive mutation lowers the critical melting temperature for the protein, thus making it denature at a lower temperature than the normal gene product (See cod.edu, 2000). A temperature-sensitive mutation makes a certain wild-type phenotype under the permissive temperature that would work and then there is a phenotype mutated that could survive in restrictive …show more content…
There are enzymes that are not functioning correctly before these compounds in which they are accumulating. In strain 136, the compound Fructose 1,6 biphosphate is collected in experiment 1 and in experiment two we see that it grew in restrictive temperature where it is also adapting to other food sources. The enzyme that is mutated to get use to its restrictive temperature is phosphofructokinase and this enzyme make its phosphate group go to the other end of the sugar. In strain 152, 3-phosphoglycerate accumulates because of the enzyme Phosphoglycerokinase helps it to grow in the restrictive temperature. The pyruvate kinase is an enzyme that is functioning incorrectly after because in the second experiment pyruvate did not accumulate and it did not grow in the unknown food source and restrictive temperature. The pyruvate strains only grew when it is added with fatty acids and heated to the restrictive temperature. I conclude that enzymes mutated could possibly stop the process of glycolysis, Krebs cycle, and electron transfer chain and different conditions could improve growth of each

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