Cellular Respiration Lab

1244 Words 5 Pages
Determining Enzymes that Result to Mutation on the three Stages of Cellular Respiration

Mary A Enriquez
University of the Pacific
November 11, 2014
2 Green
Dr. Geoffrey Lin-Cereghino

Abstract: The purpose of this lab is to determine which type of food source could possibly help strains grow on both permissive and restrictive temperature. Based on all the chemicals accumulating, we can determine the enzymes that are used to inhibit the three stages of cellular respiration.
Introduction: For cells to successfully accomplish its task such as moving and reproducing, it needs energy. Most energy comes from the sun and the energy is stored in organic molecules. Cells release its stored energy by converting chemical energy in glucose
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Cells do cellular respiration, which is divided into three different stages to convert chemical energy, cells have stored, into glucose and then make ATP. The first stage of cellular respiration is glycolysis where glucose is split into two molecules to make a compound called pyruvate. In the end of glycolysis glucose turns into two pyruvate, two ATP is made, and NAD+ formed to NADH. Below is an overview of glycolysis and the enzymes involved in the processed. Because of the pyruvate product from glycolysis, it will now enter the 2nd stage called the Citric Acid cycle, also known ask the Krebs cycle, where pyruvate is oxidize to acetyl CoA to begin the cycle. As the acetyl CoA enters it adds onto the oxaloacetate to from citric acid. The end product is 2 ATP, NADH, and …show more content…
There are enzymes that are not functioning correctly before these compounds in which they are accumulating. In strain 136, the compound Fructose 1,6 biphosphate is collected in experiment 1 and in experiment two we see that it grew in restrictive temperature where it is also adapting to other food sources. The enzyme that is mutated to get use to its restrictive temperature is phosphofructokinase and this enzyme make its phosphate group go to the other end of the sugar. In strain 152, 3-phosphoglycerate accumulates because of the enzyme Phosphoglycerokinase helps it to grow in the restrictive temperature. The pyruvate kinase is an enzyme that is functioning incorrectly after because in the second experiment pyruvate did not accumulate and it did not grow in the unknown food source and restrictive temperature. The pyruvate strains only grew when it is added with fatty acids and heated to the restrictive temperature. I conclude that enzymes mutated could possibly stop the process of glycolysis, Krebs cycle, and electron transfer chain and different conditions could improve growth of each

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