How Does Temperature Affect Enzyme Activity

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Discussion: The specific purpose for this study was to see how enzyme activity would change as there was a change in temperature and pH. Both temperature and pH were hypothesized to have an affect on enzyme activity. Each had an optimal temperature. At this point, the rate of enzyme activity would stop increasing and begin to decrease. For temperature, it was hypothesized that the optimal temperature would be 48°C. For pH, it was hypothesized that the optimal pH would be 7.
As temperature was increased, the enzyme activity also increased. Because temperature is the measure of kinetic energy, an increase in temperature would cause an increase in kinetic energy. This means that particles are moving quickly with high entropy, which causes more
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Due to this, there is a higher probability of substrates colliding with enzymes, thus making the reaction occur. This shows why enzyme activity increases with increasing temperature. However, when the optimal temperature is reached, the bonds of the enzyme begin to break apart. In an protein, various bonds, including hydrogen bonds, hold its shape together. At high temperatures, these bonds begin to break apart, which makes the enzyme unfold and lose its original shape. During the denaturing process, the precise shape needed to make enzyme reactions happen is lost. This is because the active site’s shape is specific for its substrate. Without its shape, the substrate will no longer fit. With this in mind, as temperature increases beyond its optimum, active sites are lost, and therefore the rate of reaction decreases. This is shown more clearly when the peroxidase is boiled. It is so far beyond its optimal temperature that it loses its function completely. No reactions happened in the boiled peroxidase solution. When the temperature increases this drastically beyond its optimal, the …show more content…
One error that could have taken place was failure to wipe off the outside of the test tube before placing it in the spectrophotometer. Failure to do this would make all of the absorbance levels be higher than they actually are. This error could be especially detrimental when measuring the absorbance of the 4°C peroxidase solution. The condensation on the outside of the tube would make the absorbance seem abnormally high and leveled. This could make researches believe that this temperature is more optimal than other

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