Liver Lab
By: Jonah Stillman
Background Information: Catalase enzymes are inside of nearly every living organism that is exposed to oxygen. Some examples of catalase enzymes are bacteria, plants, and animals. The main job of a catalase enzyme is to catalyze the decomposition of hydrogen peroxide to water and oxygen. When an enzyme is exposed to hydrogen peroxide or certain other chemicals it is prone to react. Catalase enzyme reactions also depend on the temperature they are exposed to. As enzymes are exposed to cool temperatures they vibrate less. They do not lose their shape when this happens, but the regions around their active active sites get frozen in place. The cool temperatures prevent the enzyme from reacting. When heated the enzyme …show more content…
(http://www.rcsb.org/pdb/101/motm.do?momID=57 -David Goodsell)
2. (http://www.ebi.ac.uk/interpro/potm/2004_9/Page1.htm -JenifferMacdowall)
Hypothesis: If we expose the catalase enzyme to freezing temperatures then the rate of the reaction will slow down. This is because when exposed to cool temperature enzymes move/vibrate less then when at regular or heated temperatures.
Experimental Design: For this experiment the independent variable is the temperature that the catalase is exposed to, and the dependent variable is the rate of the reaction. This is because the rate of the reaction depends on the temperature it is exposed to, and the rate of the reaction does not effect the temperature.
Data:
Untreated Catalase Enzyme (Controlled) …show more content…
The difference between the two graphs is the speed of the reaction. The temperatures of the two tests were very similar throughout the experiment. when doing this experiment one should have a paper towel ready to wipe the side of the test tube to be able to read the temperature.
Conclusion: This experiment tested the difference between a untreated and treated catalase enzyme. This experiment proved that as temperatures cooled the reaction time slowed down (http://www.ebi.ac.uk/interpro/potm/2004_9/Page1.htm). During the experience I initially put the thermometer on the bottom of the test tube which gave me the wrong temperature, so I had to redo the the test. Another error was during the reaction the liquid overflowed the sides of the test tube and it maid it hard to read the results. I would recommend that.
Bibliography:
1. "Catalase." Catalase Enzyme. N.p., n.d. Web. 09 Oct. 2015. .
2. "Catalase." RCSB PDB-101. N.p., n.d. Web. 09 Oct. 2015.
By: Jonah Stillman
Background Information: Catalase enzymes are inside of nearly every living organism that is exposed to oxygen. Some examples of catalase enzymes are bacteria, plants, and animals. The main job of a catalase enzyme is to catalyze the decomposition of hydrogen peroxide to water and oxygen. When an enzyme is exposed to hydrogen peroxide or certain other chemicals it is prone to react. Catalase enzyme reactions also depend on the temperature they are exposed to. As enzymes are exposed to cool temperatures they vibrate less. They do not lose their shape when this happens, but the regions around their active active sites get frozen in place. The cool temperatures prevent the enzyme from reacting. When heated the enzyme …show more content…
(http://www.rcsb.org/pdb/101/motm.do?momID=57 -David Goodsell)
2. (http://www.ebi.ac.uk/interpro/potm/2004_9/Page1.htm -JenifferMacdowall)
Hypothesis: If we expose the catalase enzyme to freezing temperatures then the rate of the reaction will slow down. This is because when exposed to cool temperature enzymes move/vibrate less then when at regular or heated temperatures.
Experimental Design: For this experiment the independent variable is the temperature that the catalase is exposed to, and the dependent variable is the rate of the reaction. This is because the rate of the reaction depends on the temperature it is exposed to, and the rate of the reaction does not effect the temperature.
Data:
Untreated Catalase Enzyme (Controlled) …show more content…
The difference between the two graphs is the speed of the reaction. The temperatures of the two tests were very similar throughout the experiment. when doing this experiment one should have a paper towel ready to wipe the side of the test tube to be able to read the temperature.
Conclusion: This experiment tested the difference between a untreated and treated catalase enzyme. This experiment proved that as temperatures cooled the reaction time slowed down (http://www.ebi.ac.uk/interpro/potm/2004_9/Page1.htm). During the experience I initially put the thermometer on the bottom of the test tube which gave me the wrong temperature, so I had to redo the the test. Another error was during the reaction the liquid overflowed the sides of the test tube and it maid it hard to read the results. I would recommend that.
Bibliography:
1. "Catalase." Catalase Enzyme. N.p., n.d. Web. 09 Oct. 2015. .
2. "Catalase." RCSB PDB-101. N.p., n.d. Web. 09 Oct. 2015.