RNA polymerase

As well as DNA, RNA molecules are also able to absorb UV light at 260nm as they contain amino acids, therefore, they both contributed to the total result. Agarose Gel Electrophoresis of DAT VNTR PCR Products DNA denatures causing a smear as when the gene is…

Heart tissue was taken out from the Refrigerator and 0.1gm was weighed. Total RNA was extracted using TRIZON Reagent according to the instructions and quantitated by Micro-spectrophotometer. RNA having a ratio of A260/280 1.9-2.0 was reversely transcribed by HiFi-MMLV cDNA kit. Quantitative real-time polymerase chain reaction was performed on a real-time fluorescent quantitative PCR system using ultra SYBR mixture with specific primers[40]. The…

single-celled organisms such as bacteria, but tend to be linear in more complex eukaryotic cells. The replicon, or the enzymatic complex that replicates DNA has limitations on its efficiency and capabilities. For example, eukaryotic DNA polymerase requires a short RNA primer to begin replication on the lagging strand, because of this replication cannot continue replication all the way to the end of the chromosome, and it has been shown that anywhere between 50 and 150 base pairs are lost from…

Abstract This paper investigates the origins, human-to-human transmission and the physiological changes in humans due to the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) and Severe Acute Respiratory Coronavirus (SARS-CoV). Research shows that these two coronaviruses are both deadly and severe their diseases are. They both have caused assorted respiratory difficulties including pneumonia, organ dysfunction, bronchitis,cough and fever, and deficiency of breath. SARS and MERS are very…

Dr. Joseph Larkin III is an associate professor in the department of Microbiology and Cell Science at the University of Florida where he teaches Immunology. In addition to teaching, Dr. Larkin oversees multiple immunology research projects involving regulatory T cells and their involvement in autoimmune diseases including type one diabetes, lupus, rheumatoid arthritis, and most recently, uveitis. Dr. Larkin was born and raised in Savannah, Georgia. When asked about how he became interested…

Several tests were accomplished technically and carefully to identify the unknown # 230 to be M.luteus bacteria. Most of the observed results do match the expected ones, except two tests. The observed results for glucose fermentation and oxidase test did not match the expected results. The expected result for fermentation of glucose test was negative while the observed result was positive. Conversely, in Kligler iron agar media, which exams the ability of bacteria to ferment lactose and glucose…

Bioinformatics is a study that focuses on the collection and analysis of biological information using information from sequences of DNA, RNA, and proteins. This field focuses on how to make predictions about biological systems and analyze data to provide more insight on how living organisms function and how its genome relates to its biology. Following the determination of the insulin sequence, Frederick Sanger realized that manually comparing several sequences was impractical (Moody 2004)).…

Agarose Gel Electrophoresis Since the discovery of the four classes of macromolecules (proteins, lipids, carbohydrates, and nucleic acids), researchers and biochemists have fine-tuned methods that reveal structural information about each of them. Different structural properties determine different functions and chemical properties, and scientists have developed intricate techniques to observe, quantify, and isolate molecules based on their aforementioned properties. Agarose gel electrophoresis,…

Gas Exchange + Transport Pulmonary circulation: circulation from heart to lungs Systemic circulation: circulation from lungs to organs Gas exchange is the movement of oxygen from the lungs to the blood and the movement of carbon dioxide from the blood to the lungs. This exchange occurs in the capillaries, which are microscopic vessels in the lungs. In humans, nutrients and oxygen are transferred through the bloodstream. Cells can’t rely solely on diffusion, so this transport system is important…

diseases outbreaks such as Smallpox, Polio, HIV/AIDS, SARS, and Ebola. One of the most latest global diseases outbreak is Ebola in West Africa. Ebola is a rare disease that caused by the Ebola virus which attack the cell body. It is a negative stranded RNA virus which derived from the Filoviridae family (3). Ebola virus can be transmitted directly through blood or other body fluids such as vomit, urine, semen, and breast milk, of infected person or indirectly by the objects that has been…