RNA interference

• The mechanism of action of cyclophosphamide is an alkylating agent that causes cross-linkage of DNA strands, thereby blocking synthesis of DNA, RNA, and protein. Has pronounced antineoplastic effects and immunosuppressive activity. EFFECTS?? • The Pharmacodynamics of the cyclophosphamide includes the absorption from the GI tack. The drug is widely distributed going to the brain, and breast milk. Should be careful while taking cyclophosphamide because it does cross the placenta. The drug…

Paclitaxel works by stopping cell division, which will kill cancer cells (Hunter, 2013, paragraph 3-6). Docetaxel also halts cell division. It works by damaging the RNA or DNA sequence that tells the cell how to copy itself. The faster the cells try to divide, the more likely the chemotherapy drugs will kill the cells, which, in turn, shrinks the tumor (ChemoCare, n.d., Section: How this drug works, Paragraph 2) Ecstasy…

The main objective of this paper by Ando et al. was to explore the nature of association between the protein MEF/ELF4 and the nucleophosmin, NPM1, along with its implications with regards to leukemogenesis; the mutant version of the latter has been commonly associated with leukemia. MEF/ELF4 is part of a family of transcriptional factors (ETS) responsible for numerous cellular functions, such as genomic stability, DNA repair, and most importantly, the regulation of cell proliferation,…

1. Name the six steps that are required to actually have a “Jurassic Park”. (6 points) 1. Find dinosaur DNA 2. Grow an embryo 3. Raise the baby 4. Choose a habitat 5. Plan a diet 6. Manage the animals 2. What was used to freeze amber to crack it open and access the insects? (1 point) Liquid nitrogen 3. Who was able to get bone marrow from fossils that have blood cells of dinosaurs in them? (1 point) John R. Horner 4. What are body cells called? (Not mentioned in video.) (2 points)…

used in biological fields, it played a huge role in the understanding and development in the knowledge about multicellular organisms since 1960s (8). Genetics and organ development, programmed cell death studies, and describing the mechanism of RNA interference (RNAi) led to award Noble prizes in physiology for Sydney Brenner, John Sulston and Robert Horvitz, Craig Mello, and Andrew Fire respectively.…

in Drosophila (DIETZL et al. 2007). In Caenorhabditis elegans (C. elegans), it is estimated that there are ~15% of the total genes in the genome are essential genes (JOHNSEN AND BAILLIE 1997), although genome-wide gene knockdown analysis using RNA interference (RNAi) only successfully identified 7% (1,170 of 16,757) defective genes that result in sterility, embryonic or larval lethality (KAMATH et al. 2003), which, however, might miss some…

In recent years the field of virology has seen substantial growth as virus have now become a more widely researched subject. It was not too long ago relatively speaking that viruses were more or less an unknown entity, as many facts about viruses were unknown to the days researchers. Now however much more is known about what viruses are and how they work; more importantly it is known that viral agents are one of the leading causes of disease. Once it was established that they did in fact…

localization pattern in Dictyostelium throughout its development will offer valuable insight into the protein’s function. Furthermore, I intend to also develop a LITAF gene knockout in Dictyostelium using various molecular techniques such as RNA interference, PCR amplification, cloning, transformation and Southern blotting. A gene knockout study will further elucidate LITAF function by allowing the identification of abnormalities during growth and development due to its gene inactivation. Dr.…

Andrew Jakymiw at Medical University of South Carolina in two independent projects. In the first project, we studied the RNA interference (RNAi) biology and how dysregulation of its molecular components contribute to oral cancer pathogenesis. We were the first group to provide biochemical evidence for the existence of the Dicer1e transcript and characterize its product, Dicer1e…

It is right for human beings to manipulate nature for their benefit. Without doing such things humans would not have lifesaving medicines or other beneficial procedures. Also, the act of altering nature is in itself a natural phenomenon, common to many species other than human beings. In addition, without the manipulation of nature we would not have anything other than the very basest of lifestyles; therefore, we would never have progressed past our ancient B.C. ancestors’ way of life. Finally,…