Reverse transcriptase

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  • Dna Synthesis Lab Report

    Objective: We started by isolating a DNA plasmid from a bacterial culture for cloning. Then, we reverse transcribed mRNA to amplify it in a polymerase chain reaction. Finally, we amplified a specific gene (actin) through PCR. Protocol: A. Isolation of Plasmid DNA from a Bacterial Culture: We were provided with a tube containing 1 ml of bacterial culture. We centrifuged it for 5 minutes at 10000 g. Then, we removed the supernatant by pouring it off and we used a small chem wipe to remove any excess media remained. Centrifuging will form a small pellet at the bottom of the tube. Using 250 l of cell resuspension solution, resuspend the cell pellet thoroughly by pipetting or vortexing. This will allow the cell lysis solution, which will be added…

    Words: 1554 - Pages: 7
  • Zika Virus Lab Report

    several complications, prompting virus detection methods to develop rapidly. (Chen, 2), (CDC) Materials and method: The detection of the ZIKV using reverse transcriptase-PCR begins with a probe and primers that were designed specifically for this experiment. FD3/FU1 forward and reverse primers were sequenced from the NS5 region that had a size of 1083bp. The primers were then amplified using plasmids and then extracted via QIAquick gel that separated them based on size. A QIAamp was then used…

    Words: 1601 - Pages: 7
  • Antibiotic Resistance Case Study

    resistance; developing new drugs and diagnosing tests can help treat the evolving bacteria and identify just exactly how it is evolving (CDC, 2015). 2. We have come a long way in the treatment and control of the spread of HIV. In a few sentences answer the questions below. Site sources. The NIH site on HIV will have most of the information you will need to answer the questions. Answer each part in 2-3 sentences. (2 points) NIH: http://www.niaid.nih.gov/topics/hivaids/Pages/Default.aspx You can…

    Words: 1374 - Pages: 5
  • Deception And Deception In Helen

    In the play Helen by Euripides the main character, Helen often finds herself in complicated situations. To get out of those situations, Helen believes that her only option is to manipulate, deceive, and lie to people. Helen’s main goal is to be reunited with her husband, Menelaos, in her homeland. Helen persuades characters to do things her way by manipulation, lies, and deception. Throughout Helen, the main character manipulates Teucros, Menelaos, and Theoclymenos using deception, persuasion,…

    Words: 1313 - Pages: 6
  • Physics Of Q-Dots Lab Report

    collected to particle size. 1 Atomic Force Microscopy Characterization In 1986, Binnig and Quate demonstrated for the first time the ideas of Atomic force microscopy, because people wanted to investigate electrically non-conductive materials.3 AFM experiments in tapping mode were performed to study the nanoparticles characterizations of that CdS-Glutathione Q-Dots sample. This is based on the image conducted using MFP-3D Origin AFM instrument (Asylum Research). For preparation, the sample…

    Words: 2135 - Pages: 9
  • Louis P. Pojman The Case Against Affirmative Action Analysis

    Louis P. Pojman argues in his article, The Case against Affirmative Action, that even in extreme cases reverse racism and strong affirmative action are wrong. Pojman differentiates between strong and weak affirmative action and in his article he focus on strong affirmative action. Pojman defines strong affirmative action as preferential treatment to someone based on race, ethnicity, or gender in favor of the under represented groups to get equal rights. The first argument made for affirmative…

    Words: 813 - Pages: 4
  • Industry Partial Survey

    An Industry Partial Survey of Operations Management Supply Chain and Logistics Issues Thomas College – Class MG 338 M Professor – Dr. Nicholas Jewczyn, Ph.D. Stanley Carte Tuesday November 25, 2014 Abstract The purpose of this paper is to better understand the writings on Six Sigma and supply chain management as it applies to operation management. A closer look will be taken at buying centers, reverse logistics and third party systems and how they effectively affect supply chain management.…

    Words: 2868 - Pages: 12
  • Affirmative Action Cons

    Before talking about affirmative action, What is affirmative action? Affirmative action is a term referring to various governments to increase the education with different races; which is saying it is trying to have other races to get into universities. Affirmative action began because of John F. Kennedy, he started affirmative action to make sure that “applicants are employed” and so the employees at the time aren't treated differently because of color or race. Which is called “Executive Order…

    Words: 889 - Pages: 4
  • Allan Bakke: Discrimination In Medical Schools

    On October 12, 1977, a thirty-five-year-old white male by the name of Allan Bakke took the University of California Davis Medical School to the California Supreme Court for rejecting his admission into the school “because of his race”; he believed his rights had been violated under the Equal Protection Clause of the Fourteenth Amendment of the U.S. Constitution, and Title VI of the Civil Rights Act of 1964. The University of California Davis Medical School reserved sixteen spots for minorities…

    Words: 531 - Pages: 3
  • Affirmative Action In Universities

    To fully understand the problems revolving around race-based admission programs in various universities, one must first understand how this issue first developed, as well as what affirmative action is. Affirmative action is a policy also known as positive discrimination, in other words, it strives to favor people who are a minority, such as, people of color. This is an important issue as it affects legislators, colleges, but most importantly future college students. For this issue, there are…

    Words: 1452 - Pages: 6
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